An activator of TRPM4
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Necrocide 1

Item No. 46570

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3-cycloheptyl-1,3-dihydro-3-(4-hydroxyphenyl)-6-methoxy-7-methyl-2H-indol-2-one
CAS Number
1247028-61-0
Synonyms
  • NC1
Molecular Formula
C23H27NO3
Formula Weight
365.5
Purity
≥98%
Formulation (Request formulation change)
A solid
SMILES
OC1=CC=C([C@]2(C3=CC=C(C(C)=C3NC2=O)OC)C4CCCCCC4)C=C1
InChi Code
InChI=1S/C23H27NO3/c1-15-20(27-2)14-13-19-21(15)24-22(26)23(19,16-7-5-3-4-6-8-16)17-9-11-18(25)12-10-17/h9-14,16,25H,3-8H2,1-2H3,(H,24,26)/t23-/m0/s1
InChi Key
JLWDEIVDLTXBGE-QHCPKHFHSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $149

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    5 mg

    $447

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    10 mg

    $745

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    Cayman Chemical
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    Product Description

    Necrocide 1 (NC1) is an activator of transient receptor potential melastatin 4 (TRPM4).1 It induces sodium influx in HEK293 cells expressing human TRPM4 at 10 µM. NC1 increases mitochondrial membrane permeability and reactive oxygen species (ROS) levels and induces necrosis in MCF-7 breast cancer cells.2 It reduces the proliferation of various cancer cell lines (IC50s = 1.57-1000 nM) but not non-cancerous cell lines (IC50s = >10,000 nM for all). NC1 induces intratumoral necrosis and decreases tumor volume in PC3 prostate cancer and MCF-7 mouse xenograft models.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Teixeira-Duarte, C.M., Fu, W., Zeng, W., et al. Structural mechanism of Necrocide 1 activation of human TRPM4 that triggers necrosis by sodium overload. Nat. Commun. 17(1), 7939 (2026).

    2. Zhang, J., Trojel-Hansen, C., Wang, J., et al. Necrocide 1 mediates necrotic cell death and immunogenic response in human cancer cells. Cell Death Dis. 14(4), 238 (2023).