Prostaglandin D₂-MOX Express ELISA Kit

Prostaglandin D₂ (PGD₂) is biosynthesized in the brain by a lipocalin-type PGD₂ synthase where it acts in the CNS to promote sleep induction and lowering of body temperature.¹ PGD₂ is also the major eicosanoid product of mast cells and is synthesized in large quantities by a hematopoetic-type PGD synthase during allergic and asthmatic anaphylaxis.^2,3 Measurement of the parent eicosanoid PGD₂ is appropriate in the supernatants of cell cultures, where PGD₂ levels may reach several ng/ml, and in CSF, where concentrations of several hundred pg/ml have been measured.⁴ Cayman Chemical’s PGD₂-MOX Express ELISA is a competitive assay that permits the rapid measurement of PGD₂ from biological samples, requiring only 1 hour incubation and development times for each step. The methodology for the assay is the same as that employed in Cayman’s widely utilized PGD₂-MOX ELISA (Item No. 512011). While Item No. 512011 offers superior sensitivity (80% B/B₀ ~3.1 pg/ml), the new PGD₂-MOX Express EIA offers the convenience of a fast assay while still achieving an 80% B/B₀ ~16 pg/ml and an assay range of 7.8-1,000 pg/ml.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

1. Hayaishi, O. Sleep-wake regulation by prostaglandins D₂ and E₂. The Journal of Biological Chemisty 263(29), 14593-14596 (1988).

2. Mahmud, I., Ueda, N., Yamaguchi, H., et al. Prostaglandin D synthase in human megakaryoblastic cells. The Journal of Biological Chemisty 272(45), 28263-28266 (1997).

3. Matsuoka, T., Hirata, M., Tanaka, H., et al. Prostaglandin D₂ as a mediator of allergic asthma. Science 287, 2013-2017 (2000).

4. Ram, A., Pandey, H.P., Matsumura, H., et al. CSF levels of prostaglandins, especially the level of prostaglandin D₂, are correlated with increasing propensity towards sleep in rats. Brain Res. 751(1), 81-89 (1997).

Kugathas, S., Audouze, K., Ermler, S., et al. Effects of common pesticides on prostaglandin D₂ (PGD₂) Inhibition in SC5 mouse sertoli cells, evidence of binding at the COX-2 active site, and implications for endocrine disruption. Environ. Health Perspect. 124(4), 452-459 (2016).

Farhat, A., Philibert, P., Sultan, C., et al. Hematopoietic-Prostaglandin D₂ synthase through PGD₂ production is involved in the adult ovarian physiology. J. Ovarian Res. 4, 3 (2011).