A fluorometric assay for the detection of thioredoxin activity in cell lysates
Features
  • Convenient 96-well plate format
  • Measure Trx activity in cell lysates
  • Active TrxR and human Trx-1 provided with kit
  • Eosin-labeled insulin used as substrate - excitation at 520 nm and emission at 560 nm
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Thioredoxin Fluorometric Activity Assay Kit

Item No. 500228

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Trx
  • Txn
Emission
560 nm
Excitation
520 nm
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    1 ea

    $578

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    Cayman Chemical
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    Product Description

    Cayman’s Thioredoxin Fluorescent Activity Assay Kit provides a convenient method of detecting Trx activity in cell lysate. Measurement of the Trx activity is based on the reduction of eosin-labeled insulin disulfides by Trx.1 The reduction of insulin disulfides results in a release of eosin and an increase in fluorescence that can be detected at 560 nm. Trx becomes oxidized during this process, and oxidized Trx is recycled back to the reduced state by Trx reductase. Under circumstances in which the Trx activity rate is limiting, the rate of fluorescence increase is directly proportional to the Trx activity in the sample.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Montano, S.J., Lu, J., Gustafsson, T.N., et al. Activity assays of mammalian thioredoxin and thioredoxin reductase: Fluorescent disulfide substrates, mechanisms, and use with tissue samples. Anal. Biochem. 449, 139-146 (2014).

    Product Citations

    Wu, D., Su, S., Zhang, P., et al. TIGAR regulates intestinal mucus barrier integrity by inhibiting lactylation of G6PD/6PGD in ulcerative colitis. Nat. Commun. 17(1), 3382 (2026).

    Camilliere, M., Verde, M.R., Rabadi, S.M., et al. Maternal undernourishment impairs murine placental development during pregnancy. BMC Pregnancy Childbirth 25(1), 889 (2025).

    Wei, Z.X., Cai, X.X., Fei, Y.D., et al. Zbtb16 increases susceptibility of atrial fibrillation in type 2 diabetic mice via Txnip‑Trx2 signaling. Cell Mol. Life Sci. 81(1), 88 (2024).

    Li, Z., LaPenna, K.B., Gehred, N.D., et al. Dysregulated protein s-nitrosylation promotes nitrosative stress and disease progression in heart failure with preserved ejection fraction. Circ. Res. 137(9), 1185-1206 (2025).