For the identification of factors regulating the phagocytic process in vitro
Features
  • Detect phagocytosis of fluorescein-labeled rabbit IgG-coated latex beads​
  • Validated in human peripheral blood monocytes and mouse peritoneal macrophages
  • Phagocytosis detectable in 1-4 hours at 37°C
  • Assess by flow cytometry or fluorescence microscopy
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Phagocytosis Assay Kit (IgG FITC)

Item No. 500290

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Cayman’s Phagocytosis Assay Kit (IgG FITC) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualizing perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough Latex Beads-Rabbit IgG-FITC Complex for up to 750 samples.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Yang, Q., Barbachano-Guerrero, A., Fairchild, L.M., et al. Macrophages derived from human induced pluripotent stem cells (iPSCs) serve as a high-fidelity cellular model for investigating HIV-1, dengue, and influenza viruses. J. Virol. 98(3), e0156323 (2024).

    Mysore, V., Cullere, X., Mears, J., et al. FcγR engagement reprograms neutrophils into antigen cross-presenting cells that elicit acquired anti-tumor immunity. Nat. Commun. 12(1), 4791 (2021).

    Pireaux, V., Sauvage, A., Bihin, B., et al. Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages. Mediators Inflamm. 2016, 8249476 (2016).

    Galleguillos, D., Wang, Q., Steinberg, N., et al. Anti-inflammatory role of GM1 and other gangliosides on microglia. J. Neuroinflammation 19(1), 9 (2026).

    Shi, G., Zhang, C., Li, G., et al. Atrazine induces phagocytotic dysfunction of microglia depends on nucleocytoplasmic translocation of acetylated HMGB1. Ecotoxicol. Environ. Saf. 252, 114583 (2023).

    Jin, M., Shiwaku, H., Tanaka, H., et al. Tau activates microglia via the PQBP1-cGAS-STING pathway to promote brain inflammation. Nat. Commun. 12(1), 6565 (2021).