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Cortisol ELISA Kit

Item № 500360
product image
     96 solid wells $219.00 0.00
     96 strip wells $219.00 0.00
     480 solid wells $876.00 0.00
     480 strip wells $876.00 0.00

Pricing updated 2019-07-21. Prices are subject to change without notice.

  • Measure cortisol in urine, plasma, and other sample matrices
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure cortisol levels down to 35 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
  • Cortisol EIA Kit

Cortisol is a glucocorticoid produced by the adrenal cortex in response to adrenocorticotropic hormone (ACTH). It is secreted with a circadian periodicity, and peaks just prior to waking in the morning. Cortisol is often elevated in major depressive disorder, certain forms of hypertension, stress, AIDS, and in the visceral fat of obese individuals. Cortisol can be measured in many matrices including blood, urine, and saliva. In serum, approximately 90-95% of cortisol is bound to proteins. Urinary cortisol is not bound to proteins, but its levels are dependent on glomerular and tubular function. In saliva, approximately 67% of cortisol is unbound. There is generally good correlation between cortisol measurements in saliva and serum. Cayman’s Cortisol ELISA Kit is a competitive assay that can be used for quantification of cortisol in urine, plasma, and other sample matrices. The assay has a range from 6.6-4,000 pg/ml and a sensitivity (80% B/B0) of approximately 35 pg/ml.

NOTE: Due to an approximate 15% cross-reactivity with dexamethasone, this kit may not be suitable for use with samples that contain high concentrations of dexamethasone, such as those collected during a dexamethasone suppression test.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Cortisol EIA Kit
Assay Range
6.6-4,000 pg/ml
80% B/B0: 35 pg/ml
50% B/B0: 150-210 pg/ml
Cross Reactivity
  • (Cortisol) 100%
  • (Prednisolone) 4.0%
  • (Cortexolone) 1.6%
Show all 14
  • Animal/Bovine
  • Animal/Eel
  • Animal/Goat
  • Animal/Mouse

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Citations

Yajima, A., Owada, H., Kobayashi, S., et al. Cacao bean husk: An applicable bedding material in dairy free-stall barns Asian-Australas. J. Anim. Sci. 30(7), 1048-1053 (2017).

Thompson, L.A., Spoon, T.R., Goertz, C.E., et al. Blow collection as a non-invasive method for measuring cortisol in the beluga (Delphinapterus leucas) PLoS One 9(12), E114062 (2014).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Cortisol ELISA Kit

View all analysis workbooks

View the pre-configured analysis tool for Cortisol ELISA Kit on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640