A fast, convenient assay format for measurement of cortisol
Features
  • Measure cortisol in urine, plasma, and other sample matrices
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Lower limit of detection - 20 pg/ml
  • Rapid assay; get results in under 4 hours
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Cortisol ELISA Kit (Express)

Item No. 500370

Kit Booklet (PDF)Validation Data (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cortisol EIA Kit
Limit of Detection
20 pg/ml
Assay Range
39.1-5,000 pg/ml
Sensitivity
80 pg/ml
Cross Reactivity
(Cortisol) 100%(Prednisolone) 4.0%(Cortexolone) 1.6%(11-Deoxycorticosterone) 0.23%(17-Hydroxyprogesterone) 0.23%(Cortisol Glucuronide) 0.15%(Corticosterone) 0.14%(Cortisone) 0.13%(Androstenedione) <0.01%(Enterolactone) <0.01%(Estrone) <0.01%(17-Hydroxypregnenolone) <0.01%(Pregnenolone) <0.01%(Testosterone) <0.01%
Origin
Animal/Bovine, Animal/Eel, Animal/Goat, Animal/Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 solid wells

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    Bioanalysis & Assay Development Services
    Your Vision. Our Expertise.
    • Bioanalysis and compound screening using Cayman or qualified commercial assay kits
    • Development and qualification of fit-for-purpose assays in a variety of formats and platforms
    • Experienced scientists dedicated to developing the best strategy for your project
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    Product Description

    Cortisol is a glucocorticoid stress hormone produced by the adrenal cortex and released in a circadian rhythm that peaks in the early morning.1,2 Because cortisol levels increase during physiological and psychological stress, cortisol is widely used as a biomarker of hypothalamic-pituitary-adrenal (HPA) axis activity.2,3 Quantification of cortisol in biological samples is commonly performed using cortisol ELISA kits for applications in stress physiology, endocrinology, metabolism, and behavioral research.

    Cayman’s Cortisol ELISA Kit (Express) is a competitive assay that can be used for the quantification of cortisol in plasma, serum, urine, saliva, feces, hair, tissue, and cell culture supernatant. The assay has a range of 39.1-5,000 pg/ml, with an average sensitivity (80% B/B0) of 80 pg/ml and a lower limit of detection (LLOD) of 20 pg/ml.

    Cortisol is identical across species that produce it; therefore, this assay can be used with samples from multiple species, including humans and other mammals.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dunlop, B.W., and Wong, A. The hypothalamic-pituitary-adrenal axis in PTSD: Pathophysiology and treatment interventions. Prog. Neuropsychopharmacol. Biol. Psychiatry 89, 361-379 (2019).

    2. James, K.A., Stromin, J.I., Steenkamp, N., et al. Understanding the relationships between physiological and psychosocial stress, cortisol and cognition. Front. Endocrinol. 14, 1085950 (2023).

    3. Wright, K.D., Hickman, R., and Laudenslager, M.L. Hair cortisol analysis: A promising biomarker of HPA activation in older adults. The Gerontologist 55(S1), S140-S145 (2015).

    Product Citations

    Jimenez, A.G., Calderaro, L., Clark, S., et al. Can dogs serve as stress mediators to decrease salivary cortisol levels in a population of liberal arts college undergraduate students? Explore (NY) 19(3), 283-289 (2023).

    Li, Z., Robaire, B., and Hales, B.F. The organophosphate esters used as flame retardants and plasticizers affect H295R adrenal cell phenotypes and functions. Endocrinology 164(9), bqad119 (2023).

    Gozdowska, M., Sokolowska, E., Pomianowski, K., et al. Melatonin and cortisol as components of the cutaneous stress response system in fish: Response to oxidative stress. Comp. Biochem. Physiol. Part A 268, 111207 (2022).

    de la Rosa, R., Vazquez, S., Tachachartvanich, P., et al. Cell-based bioassay to screen environmental chemicals and human serum for total glucocorticogenic activity. Environ. Toxicol. Chem. 40(1), 177-186 (2021).

    Tu, X., Li, Y.-W., Chen, Q.-L., et al. Tributyltin enhanced anxiety of adult male zebrafish through elevating cortisol level and distruption in serotonin, dopamine and gamma-aminobutyric acid neurotransmitter pathways. Ecotoxicol. Environ. Saf. 203, 111014 (2020).

    Martarelli, D., Cocchioni, M., Scuri, S., et al. Diaphragmatic breathing reduces exercise-induced oxidative stress. Evid. Based Complement. Alternat. Med. 932430 (2011).