STAT-8-Isoprostane ELISA Kit | Cayman Chemical
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STAT-8-Isoprostane ELISA Kit

Item № 500431
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 solid wells $250.00 0.00
     96 strip wells $250.00 0.00
     480 solid wells $938.00 0.00
     480 strip wells $938.00 0.00

Pricing updated 2018-11-14. Prices are subject to change without notice.

Description
Features
  • Measure 8-isoprostane, a biomarker of oxidative stress
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 8-isoprostane levels down to 45 pg/ml
  • Extremely rapid assay; get results in about 2 hours
  • Alkaline phosphatase used as tracer
Synonyms
  • iPF-III Alkaline Phosphatase
  • STAT-8-Isoprostane EIA Kit
  • STAT-8-epi Prostaglandin F
  • STAT-8-iso Prostaglandin F

The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. At least one of the isoprostanes, 8-isoprostane (8-iso PGF), has biological activity and has implicated as a causative mediator and specific marker of free radical-induced damage in a variety of diseases related to oxidative stress.1,2,3,4 Cayman Chemical's STAT-8-isoprostane ELISA is a competitive assay that permits the rapid measurement of 8-iso PGF from biological samples, requiring only 1 hour incubation and development times for each step. This assay format is the similar to that employed in Cayman's popular 8-isoprostane ELISA (Item No. 516351) with the only change being the use of an alkaline phosphatase tracer in place of an AChE tracer. While Item No. 516351 offers superior sensitivity (IC50 ~35 pg/ml), the new STAT-8-isoprostane Assay offers the convenience of a fast assay while still achieving an IC50 of 180 pg/ml and a detection limit (80% B/B0) of approximately 45 pg/ml.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • iPF-III Alkaline Phosphatase
  • STAT-8-Isoprostane EIA Kit
  • STAT-8-epi Prostaglandin F
  • STAT-8-iso Prostaglandin F
Assay Range
23.4-3,000 pg/ml
Sensitivity
80% B/B0: 45 pg/ml
Mid-point
50% B/B0: 100-300 pg/ml
Cross Reactivity
  • (Prostaglandin D2) 0.16%
  • (6-keto Prostaglandin F) 0.14%
  • (Prostaglandin F) 0.14%
Show all 22

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Banerjee, M., Kang, K.H., Morrow, J.D., et al. Effects of a novel prostaglandin, 8-epi-PGF, in rabbit lung in situ American Journal of Physiology 263, H660-H663 (1992).

2. Vacchiano, C.A., and Tempel, G.E. Role of nonenzymatically generated prostanoid, 8-iso-PGF, in pulmonary oxygen toxicity Journal of Applied Physiology 77, 2912-2917 (1994).

3. Praticò, D., Lawson, J.A., Rokach, J., et al. The isoprostanes in biology and medicine Trends in Endocrinology and Metabolism 12(6), 243-247 (2001).

4. Greco, A., Minghetti, L., and Levi, G. Isoprostanes, novel markers of oxidative injury, help understanding the pathogenesis of neurodegenerative diseases Neurochemical Research 25(9/10), 1357-1364 (2000).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for STAT-8-Isoprostane ELISA Kit

View all analysis workbooks

View the pre-configured analysis tool for STAT-8-Isoprostane ELISA Kit on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of STAT-8-Isoprostane ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640