A colorimetric assay for the quantification of creatinine in urine
Features
  • Measure creatinine levels in urine
  • Assay 40 samples in duplicate
  • Measure creatinine levels down to 0.1 mg/dl
  • Plate-based colorimetric measurement (490-500 nm)
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Creatinine (urinary) Colorimetric Assay Kit

Item No. 500701

Technical Information
Assay Range
0-15 mg/dl
Shipping & Storage Information
Storage
Room temperature
Shipping
Ambient in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    Add

    Add

    Bioanalysis & Assay Development Services
    Your Vision. Our Expertise.
    • Bioanalysis and compound screening using Cayman or qualified commercial assay kits
    • Development and qualification of fit-for-purpose assays in a variety of formats and platforms
    • Experienced scientists dedicated to developing the best strategy for your project
    LEARN MORE
    Product Description

    Cayman’s Creatinine (urinary) Colorimetric Assay Kit can be used to measure creatinine levels in urine. The assay relies on the Jaffe reaction, wherein a yellow/orange color forms when the metabolite is treated with alkaline picrate.1 The color derived from creatinine is then destroyed at acidic pH. The difference in color intensity measured at 500 nm before and after acidification is proportional to the creatinine concentration.2,3,4 The sample creatinine concentration is determined using a creatinine standard curve with a range of 0-15 mg/dl.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Slot, C. Plasma creatinine determination. A new and specific Jaffe reaction method. Scand. J. Clin. Lab. Invest. 17(4), 381-387 (1965).

    2. Bowers, L.D., and Wong, E.T. Kinetic serum creatinine assays. II. A critical evaluation and review. Clin. Chem. 26(5), 555-561 (1980).

    3. Heinegård, D., and Tiderström, G. Determination of serum creatinine by a direct colorimetric method. Clin. Chim. Acta 43(3), 305-310 (1973).

    4. Cook, J.G.H. Factors influencing the assay of creatinine. Ann. Clin. Biochem. 12(6), 219-232 (1975).

    Product Citations

    Ontawong, A., Nehra, G., Maloney, B.J., et alN-acetylcysteine attenuates Aβ-mediated oxidative stress, blood-brain barrier leakage, and renal dysfunction in 5xFAD mice. Int. J. Mol. Sci. 26(9), 4352 (2025).

    Lambooy, S., Heida, A., Joschko, C., et alSelective hepatic Cbs knockout aggravates liver damage, endothelial dysfunction and ROS stress in mice fed a western diet. Int. J. Mol. Sci. 24(8), 7019 (2023).

    Vieira, G.d.L.T., Lossie, A.C., Lay, D.C., Jr., et alPreventing, treating, and predicting barbering: A fundamental role for biomarkers of oxidative stress in a mouse model of Trichotillomania. PLoS One 12(4), e0175222 (2017).

    Vieira, G.L.T., Lossie, A.C., Lay, D.C., Jr., et alPreventing, treating, and predicting barbering: A fundamental role for biomarkers of oxidative stress in a mouse model of Trichotillomania. PLoS One 12(4), e0175222 (2017).

    Withee, E.D., Tippens, K.M., Dehen, R., et alEffects of methylsulfonylmethane (MSM) on exercise-induced oxidative stress, muscle damage, and pain following a half-marathon: a double-blind, randomized, placebo-controlled trial. J. Int. Soc. Sports Nutr. 14:24, (2017).

    Wood, H.E., Marlin, N., Mudway, I.S., et alEffects of air pollution and the introduction of the London low emission zone on the prevalence of respiratory and allergic symptoms in schoolchildren in East London: A sequential cross-sectional study. PLoS One 10(8), e0109121 (2015).

    Li, H., Liu, K., Boardman, L.A., et alCirculating prostaglandin biosynthesis in colorectal cancer and potential clinical significance. EBioMedicine 2(2), 165-171 (2015).

    Dhakal, K., Uwimana, E., Adamcakova-Dodd, A., et alDisposition of phenolic and sulfated metabolites after inhalation exposure to 4-chlorobiphenyl (PCB3) in female rats. Chem. Res. Toxicol. 27(8), 1411-1420 (2014).

    Claybaugh, T., Decker, S.J., McCall, K., et alL-Arginine supplementation in type II diabetic rats preserves renal function and improves insulin sensitivity by altering the nitric oxide pathway. Int. J. Endocrinol. 171546 (2014).

    Gilbert-Diamond, D., Li, Z., Perry, A.E., et alA population-based case-control study of urinary arsenic species and squamous cell carcinoma in New Hampshire, USA. Environ. Health Perspect. 121(10), 1154-1160 (2013).

    Addabbo, F., Ratliff, B., Park, H.-C., et alThe Krebs cycle and mitochondrial mass are early victims of endothelial dysfunction. Am. J. Pathol. 174(1), 34-43 (2009).