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Cayman’s Creatinine (urinary) Colorimetric Assay Kit can be used to measure creatinine levels in urine. The assay relies on the Jaffe reaction, wherein a yellow/orange color forms when the metabolite is treated with alkaline picrate.1 The color derived from creatinine is then destroyed at acidic pH. The difference in color intensity measured at 500 nm before and after acidification is proportional to the creatinine concentration.2,3,4 The sample creatinine concentration is determined using a creatinine standard curve with a range of 0-15 mg/dl.
Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.
WARNING This product is not for human or veterinary use.
1. Plasma creatinine determination. A new and specific Jaffe reaction method. Scand. J. Clin. Lab. Invest. 17(4), 381-387 (1965).
2. Kinetic serum creatinine assays. II. A critical evaluation and review. Clin. Chem. 26(5), 555-561 (1980).
3. Determination of serum creatinine by a direct colorimetric method. Clin. Chim. Acta 43(3), 305-310 (1973).
4. Factors influencing the assay of creatinine. Ann. Clin. Biochem. 12(6), 219-232 (1975).
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