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PAD4 Autoantibody ELISA Kit

Item № 500930
product image
     96 wells $425.00 0.00

Pricing updated 2019-07-18. Prices are subject to change without notice.

  • Measure anti-PAD4 autoantibodies of any isotype (IgM, IgG, IgA)
  • For use in human plasma and serum (no need to purify samples)
  • Assay range: 15.6-1,000 U/ml with an LLOQ of 31.3 U/ml
  • PAD4 Autoantibody EIA Kit
  • PADI4
  • PADI5
  • PDI5
  • Peptidylartginine Deaminase 4
  • Protein Arginine Deiminase 4

Protein arginine deiminase 4 (PAD4) is a guanidino-modifying enzyme which catalyzes the conversion of specific arginine residues to citrulline in a calcium-dependent manner. PAD4 activity is increased in rheumatoid arthritis (RA), producing citrulline-containing proteins (CCP) that induce the formation of arthritogenic autoantibodies. In addition to anti-CCP autoantibodies, 23-45% of RA patients also produce autoantibodies specific for the PAD4 enzyme itself. Cayman’s PAD4 Autoantibody ELISA Kit is an immunometric assay which can be used to measure anti-PAD4 autoantibodies of any isotype (IgM, IgG, and IgA) in human plasma and serum without prior sample purification. Affinity-purified anti-PAD4 antibody isolated from the plasma of a patient with RA is used as the standard. One unit is approximately equal to 1 ng of anti-PAD4 Ig protein. The standard curve spans the range of 15.6-1,000 U/ml, with an LLOQ of 31.3 U/ml.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • PAD4 Autoantibody EIA Kit
  • PADI4
  • PADI5
  • PDI5
  • Peptidylartginine Deaminase 4
  • Protein Arginine Deiminase 4
Assay Range
15.6-1,000 U/ml
Lower Limit of Quantification
31.3 U/ml
  • Animal/Bovine
  • Animal/Goat

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Citations

Yadav, R., Yoo, D., Kahlenberg, J.M., et al. Systemic levels of anti-PAD4 autoantibodies correlate with airway obstruction in cystic fibrosis J. Cyst. Fibros. (2019).

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