A competitive assay for the quantification of tetranor-PGDM in urine
Features
  • Detect tetranor-PGDM, a major metabolite of PGD2 in urine
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure tetranor-PGDM levels down to 40 pg/ml
  • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
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tetranor-PGDM ELISA Kit

Item No. 501001

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • tetranor-PGD Metabolite
  • tetranor-Prostaglandin D Metabolite EIA Kit
Assay Range
6.4-4,000 pg/ml
Sensitivity
80% B/B0: 40 pg/ml
Cross Reactivity
(tetranor-PGDM) 100%(tetranor-PGJM) 100%(tetranor-PGAM) 2.08%(tetranor-PGEM) 0.03%(tetranor-PGFM) <0.01%(Prostaglandin A2) <0.01%(Prostaglandin D2) <0.01%(13,14-dihydro-15-keto Prostaglandin D2) <0.01%(Prostaglandin E2) <0.01%(13,14-dihydro-15-keto Prostaglandin E2) <0.01%(Prostaglandin F2α) <0.01%(13,14-dihydro-15-keto Prostaglandin F2α) <0.01%(Prostaglandin J2) <0.01%
Origin
Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    tetranor-PGDM is a major metabolite of prostaglandin D2 (PGD2) found in human and mouse urine.1 In human urine, tetranor-PGDM is significantly more abundant than the PGD2 metabolites 11β-PGF2α and 2,3-dinor-11β-PGF2α and is the only endogenous PGD2 metabolite detectable in mouse urine by LC-MS. Normal levels of tetranor-PGDM in human and mouse urine are 1.5 ng/mg creatinine and 8.1 ng/mg creatinine respectively.1 Cayman’s tetranor-PGDM ELISA Kit is a competitive assay that can be used for quantification of tetranor-PGDM in urine. This assay converts tetranor-PGDM to a stable derivative, tetranor-PGJM, that can be easily quantified. The assay has a range from 6.4-4,000 pg/ml and a sensitivity (80% B/B0) of approximately 40 pg/ml.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Song, W.L., Wang, M., Ricciotti, E., et al. Tetranor PGDM, an abundant urinary metabolite reflects biosynthesis of prostaglandin D2 in mice and humans. The Journal of Biological Chemisty 283(2), 1179-1188 (2008).