Vitamin D ELISA Kit

The primary synthesis of functional vitamin D begins in the skin, where a cholesterol by-product acted upon by UV light forms vitamin D₃. Vitamin D₃ is carried through the bloodstream, attached to the vitamin D binding protein, to the liver where it is converted to 25-hydroxy vitamin D₃ (25(OH)D₃). This product is then transported to the kidney where it is further converted to 1,25-dihydroxycholecalciferol (1,25(OH)₂D₃), the biologically active form. Active 1,25(OH)₂D₃ is very short-lived and is rapidly metabolized to its deactivated forms.

Vitamin D Immunoassay:
Accurate Vitamin D measurement in plasma or serum
Cayman’s Vitamin D EIA Kit is a competitive assay that can be used for Vitamin D quantification in plasma or serum. Because of the short half-life of the biologically active 1,25(OH)D form, this Vitamin D assay primarily detects the more metabolically stable forms, 25(OH)D₃ and 25(OH)D₂. Accurate detection of these forms requires that they be displaced from the vitamin D binding protein prior to measurement. A simple, acetone-based purification protocol is included as the initial procedure of the Vitamin D assay kit for this purpose.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

Krejner, A., Litwiniuk, M., and Grzela, T. LL-37 but not 25-hydroxy-vitamin D serum level correlates with healing of venous leg ulcers. Arch. Immunol. Ther. Exp. (Warsz.) 65(5), 455-461 (2016).

Zhang, L., Chen, X., Qu, J., et al. The effects of different intensities of exercise and active vitamin D on mouse bone mass and bone strength. J. Bone Miner. Metab. 35(3), 265-277 (2016).