For the quantification of DHN-MA in urine samples
Features
  • Measure DHN-MA, a HNE metabolite, in urine samples
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Incubation: Overnight
  • Species Compatibility: Human | Mouse | Rat | Dog | Pig
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DHN-MA EIA Kit

Item No. 501140

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 1,4-Dihydroxynonane Mercapturic Acid ELISA
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $778

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    This product has been produced by

    Precellys
    Bioanalysis & Assay Development Services
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    • Bioanalysis and compound screening using Cayman or qualified commercial assay kits
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    Product Description

    Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Measurement of 4-hydroxy-2-nonenal (HNE), an aldehyde formed during the lipid peroxidation process, is one of several accepted biomarkers for lipid peroxidation. 1,4-Dihydroxynonane mercapturic acid (DHN-MA) is the major urinary metabolite of HNE present at physiological levels in rat and human urine. It can be measured without extraction and offers an alternative lipid peroxidation biomarker aside from 8-isoprostane or TBARS. This DHN-MA EIA Kit is a competitive assay that can be used for quantification of DHN-MA in urine samples. The EIA typically displays an IC50 (50% B/B0) of approximately 75 pg/ml and a detection limit (80% B/B0) of approximately 10 pg/ml. [Bertin Catalog No. A05185]

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    De Filippo, C., Chiccioli, S., Meriggi, N., et al. Gut microbiota drives colon cancer risk associated with a diet: A comparative analysis of meat-based and pesco-vegetarian diets. Microbiome 12, 180 (2024).

    Guéraud, F., Buisson, C., Promeyrat, A., et al. Effects of sodium nitrite reduction, removal or replacement on cured and cooked meat for microbiological growth, food safety, colon ecosystem, and colorectal carcinogenesis in Fischer 344 rats. NPJ Sci. Food 7, 53 (2023).

    Rigby, M.J., Lawton, A.J., Kaur, G., et al. Endoplasmic reticulum acetyltransferases Atase1 and Atase2 differentially regulate reticulophagy, macroautophagy and cellular acetyl-CoA metabolism. Commun. Biol. 4(1), 454 (2021).