A competitive assay for the quantification of corticosterone in serum and rodent feces
Features
  • Measure corticosterone in serum and rodent feces
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure corticosterone levels down to 30 pg/ml
  • Incubation: Overnight | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
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Corticosterone ELISA Kit

Item No. 501320

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Corticosterone EIA Kit
  • 17-deoxy Cortisol
  • 11β,21-DHP
  • 11β,21-dihydroxy Progesterone
Assay Range
8.2-5,000 pg/ml
Sensitivity
80% B/B0: 30 pg/ml
Cross Reactivity
(Corticosterone) 100%(11-Deoxycorticosterone) 15.8%(Prednisolone) 3.4%(11-Dehydrocorticostone) 2.9%(Cortisol) 2.5%(Progesterone) 1.4%(Aldosterone) 0.47%(17α-Hydroxyprogesterone) 0.21%(11-Deoxycortisol) 0.14%(Androstenedione) 0.11%(Testosterone) 0.07%(Pregnenolone) 0.03%(DHEA sulfate) 0.0005%(Androstenediol) <0.01%(Dexamethasone) <0.01%(DHEA) <0.01%(5α-DHT) <0.01%(Estradiol) <0.01%(Estriol) <0.01%(Estrone sulfate) <0.01%(Ethynylestradiol) <0.01%(17α-Hydroxypregnenolone) <0.01%
Origin
Animal/Bovine, Animal/Eel, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Bioanalysis & Assay Development Services
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    Product Description

    Corticosterone is a glucocorticoid produced by the adrenal cortex in response to ACTH (corticotropic hormone) and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress; therefore, corticosterone can be used as a biomarker of stress.1 Biologists often measure corticosterone in fecal material of the species they are studying.1,2 This non-invasive sample collection procedure has the advantage of not itself causing stress and thereby increasing corticosterone levels, a common problem when samples for measurement of corticosterone are collected by more invasive means.1 Plasma corticosterone levels have a circadian variation and corticosterone may be important in the regulation of the sleep-wake cycle.2,3 Cayman’s Corticosterone ELISA Kit is a competitive assay that can be used for quantification of corticosterone in serum and rodent feces.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Goymann, W., Möstl, E., and Gwinner, E. Corticosterone metabolites can be measured noninvasively in excreta of European stonechats (Saxicola torquata rubicola). Auk 119(4), 1167-1173 (2002).

    2. Vázquez-Palacios, G., Retana-Márquez, S., Bonilla-Jaime, H., et al. Further definition of the effect of corticosterone on the sleep-wake pattern in the male rat. Pharmacol. Biochem. Behav. 70(2-3), 305-310 (2001).

    3. Millspaugh, J.J., Washburn, B.E., Milanick, M.A., et al. Effects of heat and chemical treatments on fecal glucocorticoid measurements: Implications for sample transport. Wildl. Soc. Bull. 31(2), 399-406 (2003).

    Product Citations

    Wang, X., Sun, Z., Yang, T., et al. Sodium butyrate facilitates CRHR2 expression to alleviate HPA axis hyperactivity in autism-like rats induced by prenatal lipopolysaccharides through histone deacetylase inhibition. mSystems 8(4), e0041523 (2023).

    Neely, W.J., Martins, R.A., Mendonça da Silva, C.M., et al. Linking microbiome and stress hormone responses in wild tropical treefrogs across continuous and fragmented forests. Commun. Biol. 6(1), 1261 (2023).

    Greene, S.M., Klein, P.R., Alcala, G.A., et al. Aging to 24 months increased C57BL/6J mouse social sniffing and hippocampal Neto1 levels, and impaired female spatial learning. Behav. Processes 211:104929, (2023).

    A.C., d.F., Nogueira, L.A.K., Titon, S.C.M., et al. Immune and hormonal regulation of the Boa constrictor (Serpentes; Boidae) in response to feeding. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 264, 111119 (2022).

    Koike, T., Yoshikawa, M., Ando, H.K., et al. Discovery of soticlestat, a potent and selective inhibitor for cholesterol 24-hydroxylase (CH24H). J. Med. Chem. 64(16), 12228-12244 (2021).

    Leishman, E.M., van Staaveren, N., Mohr, J., et al. The effect of egg laying on feather and plasma corticosterone concentrations in turkey (Meleagris gallopavo) hens. Animals (Basel) 11(7), 1892 (2021).

    Gokdemir, O., Cetinkaya, C., Gumus, H., et al. The effect of exercise on anxiety- and depression-like behavior of aged rats. Biotech. Histochem. 95(1), 8-17 (2020).

    Li, N., Xu, N., Lin, Y., et al. Roles of testosterone and estradiol in mediation of acute neuroendocrine and electroencephalographic effects of sevoflurane during the sensitive period in rat. Front. Endocrinol. (Lausanne) 11, 545973 (2020).

    Forsburg, Z.R., Goff, C.B., Perkins, H.R., et al. Validation of water-borne cortisol and corticosterone in tadpoles: Recovery rate from an acute stressor, repeatability, and evaluating rearing methods. Gen. Comp. Endocrinol. 281, 145-152 (2019).

    Fabrício-Neto, A., Madelaire, C.B., Gomes, F.R., et al. Exposure to fluctuating temperatures leads to reduced immunity and to stress response in rattlesnakes. J. Exp. Biol. 222(Pt 22), jeb208645 (2019).

    Hammond, T.T., Vo, M., Burton, C.T., et al. Physiological and behavioral responses to anthropogenic stressors in a human-tolerant mammal. J. Mammal. 100(6), 1928–1940 (2019).

    Bartz, B.M., McIntyre, D.R., and Grimes, J.L. Effects of management related practices on turkey hen performance supplemented with either Original XPC™ or AviCare™. Front. Vet. Sci. 5, 185 (2018).

    Li, A., Zhang, R.-X., Wang, Y., et al. Corticosterone mediates electroacupuncture-produced anti-edema in a rat model of inflammation. BMC Complement. Altern. Med. 7(27), (2007).

    Borel, C., Welti, D.H., Fernandez, I., et al. Dicranin, an antimicrobial and 15-lipoxygenase inhibitor from the moss Dicranum scoparium. J. Nat. Prod. 56(7), 1071-1077 (1993).