For the quantification of sPLA2 in plasma, synovial fluid, and other sample matrices
Features
  • Measure sPLA2 levels in plasma, synovial fluid, and other sample matrices
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Assay Range: 31.3-2,000 pg/ml with an LLOQ of 31.3 pg/ml
  • Rapid assay; get results in under 4 hours
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sPLA2 (human Type IIA) ELISA Kit

Item No. 501380

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Secretory Phospholipase A2 (human synovial) EIA Kit
Assay Range
31.3-2,000 pg/ml
Lower Limit of Quantification
31.3 pg/ml
Origin
Animal/Bovine, Animal/Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $549

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    Product Description

    sPLA2s are found in the venom of certain snakes (Types IA, IIA, and IIB from vipers, cobras, rattlesnakes, and kraits), in pancreatic juices (Type IB), in rat and murine testes (Type IIC), in placenta, synovial fluids and platelets (Type IIA), and in heart, placenta, lung, mast cells, and P388D1 macrophages (Type V).1 Until 1994, the sPLA2 responsible for the release of arachidonic acid in inflammation was believed to be Type IIA. Although recently discovered isoforms of PLA2 clearly contribute to the release of arachidonic acid, sPLA2 (Type IIA) continues to be a protein of interest in the field of inflammation. This ELISA is specific for Type IIA sPLA2, and does not cross react with Type I, Type IV, Type V PLA2, inflammatory mediators such as tumor necrosis factor, interleukin-1, or platelet-activating factor. Cayman’s sPLA2 (human Type IIA) ELISA Kit is an immunometric assay that can be used for quantification of sPLA2 in plasma, synovial fluid, and other sample matrices.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dennis, E.A. The growing phospholipase A2 superfamily of signal transduction enzymes. Trends Biochem. Sci. 22(1), 1-2 (1997).

    Product Citations

    Tan, T.L., Ahmad, N.S., Nasuruddin, D.N., et al. CD64 and group II secretory phospholipase A2 (sPLA2-IIA) as biomarkers for distinguishing adult sepsis and bacterial infections in the emergency department. PLoS ONE 11(3), e0152065 (2016).

    Romero-Estrada, A., Maldonado-Magaña, A., González-Christen, J., et al. Anti-inflammatory and antioxidative effects of six pentacyclic triterpenes isolated from the Mexican copal resin of Bursera copallifera. BMC Complement. Altern. Med. 16(1), 422 (2016).

    Mearelli, F., Fiotti, N., Altamura, N., et al. Heterogeneous models for an early discrimination between sepsis and non-infective SIRS in medical ward patients: A pilot study. Intern. Emerg. Med. 9, 749-757 (2014).

    Wootton, P.T.E., Arora, N.L., Drenos, F., et al. Tagging SNP haplotype analysis of the secretory PLA2-V gene, PLA2G5, shows strong association with LDL and oxLDL levels, suggesting functional distinction from sPLA2-IIA: Results from the UDACS study. Hum. Mol. Genet. 16(12), 1437-1444 (2007).