A competitive ELISA for the quantification of (±)12(13)-DiHOME in plasma, serum and BALF
Features
  • Measures (±)12(13)-DiHOME
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure (±)12(13)-DiHOME levels down to 0.05 ng/ml
  • Incubation: 2 hours | Development: 90 minutes | Read: Colorimetric at 414 nm
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(±)12(13)-DiHOME ELISA Kit

Item No. 501720

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • (±)12(13)-DiHOME EIA Kit
  • 12,13-dihydroxy-9Z,Octadecenoic Acid
  • Isoleukotoxin
Limit of Detection
0.043 ng/ml
Assay Range
0.05-100 ng/ml
Sensitivity
0.289 ng/ml
Cross Reactivity
(100%) (±)12(13)-DiHOME(0.30%) (±)9(10)-DiHODE(0.16%) (±)12(13)-EpOME(0.03%) 9-OxoODE(0.03%) (±)13-HpODE(0.02%) 5(S)-HETE(0.02%) (±)11(12)-EET(0.02%) (±)14(15)-EET(<0.03%) (±)12-HETE(<0.03%) (±)9-HODE
Origin
Animal/Bovine, Animal/Eel, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 solid wells

    $504

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    96 strip wells

    $504

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    Product Description

    Cayman’s (±)12(13)-DiHOME ELISA Kit is a competitive assay that can be used for quantification of 12(13)-DiHOME in plasma, serum, and bronchoalveolar lavage fluid (BALF). The assay has a range of 0.05-100 ng/ml and a sensitivity (80% B/B0) of approximately 0.3 ng/ml.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Nirengi, S., Buck, B., Das, D., et al. 12,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKII. Nat. Commun. 16(1), 7088 (2025).