A competitive ELISA for the quantification of testosterone glucuronide
Features
  • Measure testosterone glucuronide, the predominant urinary metabolite of testosterone, in biological samples
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Lower limit of detection (LLOD) is 8.9 pg/ml
  • Incubation: 18 hours | Development: 90 minutes | Read: Colorimetric at 405 nm
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Testosterone Glucuronide ELISA Kit

Item No. 501740

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Testosterone Glucuronide EIA Kit
Limit of Detection
8.9 pg/ml
Assay Range
4.9-3,000 pg/ml
Sensitivity
25 pg/ml
Cross Reactivity
(30.8%) Testosterone(9.9%) Progesterone(9.5%) Androstenedione(2.2%) epi-Testosterone(1.9%) Nandrolone(1.2%) Corticosterone(0.8%) 11-keto Testosterone(0.5%) Aldosterone(0.1%) Cortisol(0.07%) epi-Testosterone Glucuronide(0.02%) Androsterone(0.01%) Pregnanediol-1-Glucuronide(<0.01%) DHEA Sulfate(<0.01%) Estradiol 17-(β-Glucuronide)(<0.01%) Estradiol 17-Sulfate
Origin
Animal/Bovine, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    96 solid well

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    96 strip well

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    Product Description

    Testosterone glucuronide is a urinary metabolite of the endogenous steroid hormone testosterone.1 It is formed by glucuronide conjugation of testosterone primarily by the UDP-glucuronosyltransferase (UGT) isoform UGT2B17, which is found in the small intestine, colon, and liver.1,2,3

    Testosterone glucuronide levels are lower in individuals with mutations in the UGT2B17 gene.4 Glucuronidation of testosterone is decreased by the NSAIDs diclofenac and ibuprofen in vitro, but not in vivo.5,6

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Baulieu, E.-E., and Mauvais-Jarvis, P. Studies on testosterone metabolism. The Journal of Biological Chemisty 239(5), 1569-1577 (1964).

    2. Zhang, H., Basit, A., Busch, D., et al. Quantitative characterization of UDP-glucuronosyltransferase 2B17 in human liver and intestine and its role in testosterone first-pass metabolism. Biochem. Pharmacol. 156, 32-42 (2018).

    3. Fagerberg, L., Hallstrom, B.M., Oksvold, P., et al. Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics. Mol. Cell. Proteomics 13(2), 397-406 (2014).

    4. Schulze, J.J., Lundmark, J., Garle, M., et al. Doping test results dependent on genotype of uridine diphospho-glucuronosyl transferase 2B17, the major enzyme for testosterone glucuronidation. J. Clin. Endocrinol. Metab. 93(7), 2500-2506 (2008).

    5. Sten, T., Finel, M., Ask, B., et al. Non-steroidal anti-inflammatory drugs interact with testosterone glucuronidation. Steroids 74(12), 971-977 (2009).

    6. Lundmark, J., Garevik, N., Thomgren, J.-O., et al. Non-steroidal anti-inflammatory drugs do not influence the urinary testosterone/epitestosterone glucuronide ratio. Front. Endocrinol. (Lausanne) 4:51, (2013).