A competitive ELISA for the quantification of cyclic di-GMP
Features
  • Measure cyclic di-GMP in bacterial lysates
  • Lower limit of detection (LLOD) is 5.3 pg/ml (7.7 fmol/ml or 7.7 pM)
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Rapid assay; get results in under 4 hours
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Cyclic di-GMP ELISA Kit

Item No. 501780

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • c-di-GMP EIA
  • Cyclic Diguanosine-5'-monophosphate
  • Cyclic Diguanylate
  • 3',5'-Cyclic Diguanylic Acid
Limit of Detection
5.3 pg/ml (7.7 pM)
Assay Range
4.6-10,000 pg/ml (6.7-14,480 pM)
Sensitivity
30 pg/ml (43 pM)
License
This product was developed in conjunction with BIOLOG Life Science Institute.
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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    96 solid wells

    $559

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    96 strip wells

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    Product Description

    Cayman’s Cyclic di-GMP ELISA Kit is a competitive assay that can be used for quantification of cyclic di-GMP in bacterial cell lysates. The assay has a range of 4.6-10,000 pg/ml (6.7-14,480 pM) with an average sensitivity (80% B/B0) of 30 pg/ml (43 pM) and a Lower Limit of detection (LLOD) of 5.3 pg/ml (7.7. pM). Powered by BIOLOG Life Science Institute.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    de Dios, R., Forsyth, V.S., Harkova, L.G., et al. Network-level divergence in cyclic di-GMP signalling drives ecological versatility in Acinetobacter baumannii. NPJ Biofilms Microbiomes (2026).

    Beckman, R.L., IV, Martinez, B., Santiago, F.Z., et al. Molecular response to the non-lytic peptide bac7 (1-35) triggers disruption of Klebsiella pneumoniae biofilm. PLoS Pathog. 21(12), e1013437 (2025).

    Costello, C., Murphree-Terry, M., Oden, A., et al. Pseudomonas aeruginosa increases viscoelasticity and decreases transportability of artificial mucus. IScience 28(9), 113265 (2025).

    Tronnet, S., Pandey, V., Lloret-Berrocal, M., et al. Extracellular ATP is an environmental cue in bacteria. Cell Rep. 44(10), 116356 (2025).

    Yang, J.R., Chen, K.T., Chen, Y.W., et al. The diguanylate cyclase and phosphodiesterase locally regulate the virulence factors in Vibrio vulnificus. Gut Pathog. 17(1), 87 (2025).

    Isenberg, R.Y., Holschbach, C.S., Gao, J., et al. Functional analysis of cyclic diguanylate-modulating proteins in Vibrio fischeri. mSystems 9(11), e0095624 (2024).