A competitive ELISA for the quantification of estradiol in plasma and serum
Features
  • Measure estradiol in plasma and serum samples
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure estradiol levels down to 20 pg/ml
  • Incubation: 2 hours | Development: 60 minutes | Read: Colorimetric at 414 nm
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Estradiol ELISA Kit

Item No. 501890

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Estradiol EIA Kit
  • E2
  • β-Estradiol
  • 17β-Estradiol
  • 17β-Oestradiol
Limit of Detection
6 pg/ml
Assay Range
0.61-10,000 pg/ml
Sensitivity
20 pg/ml
Cross Reactivity
(100%) Estradiol(2.5%) Methoxyestradiol(2.3%) Estradiol 3-(β-D-Glucuronide)(1.38%) Estrone(1.3%) 2-Hydroxyestradiol(1.0%) Estriol(0.7%) Estradiol Benzoate(0.53%) Estradiol 3-sulfate(0.14%) Ethynyl Estradiol(0.06%) 5-Androstan-17β-ol-3-one(0.04%) 17α-Estradiol(0.04%) 5α-dihydro Testosterone(0.03%) Androstenediol(0.03%) Testosterone(0.02%) Estradiol 17-sulfate(0.02%) Estradiol 17-(β-D-Glucuronide)(<0.01%) Aldosterone(<0.01%) Cortisol(<0.01%) DHEA(<0.01%) DHEA Sulfate(<0.01%) Maturation-Inducing Steroid (salmonid)(<0.01%) Progesterone
Origin
Animal/Bovine, Animal/Eel, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    Product Description

    Estradiol is a steroid hormone produced from testosterone via the aromatase system in the granulosa cells of ovarian follicles.1,2 It is instrumental in the development of secondary sex characteristics at puberty and in the menstrual cycle.3,4,5 Plasma levels of estradiol peak during the follicular phase of the menstrual cycle to approximately 300 pg/ml.1,4,5 During this time, it stimulates proliferation of granulosa cells, increases the size of uterine glands, and exerts positive feedback on the hypothalamus, leading to an increase in luteinizing hormone.5,6 Blood levels of estradiol drop as luteinizing hormone levels increase and trigger ovulation, then rise again during the luteal phase to approximately 100 pg/ml.4 Estradiol is metabolized into estrone by 17β-hydroxysteroid dehydrogenase 2 and hydroxylated metabolites such as estriol, as well as glucuronidated and sulfonated metabolites, which are excreted in the urine and feces.7

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Williams, C.J., and Erickson, G.F. Morphology and physiology of the ovary. Endotext [Internet] (2000).

    2. Vance, D.E. Cholesterol and related derivatives. Biochemistry 2nd ed., 725-748 (1988).

    3. Bordini, B., and Rosenfield, R.L. Normal pubertal development: Part I: The endocrine basis of puberty. Pediatr. Rev. 32(6), 223-229 (2011).

    4. Jones, R.E., and Lopez, K.H. Chapter 3 - The Menstrual Cycle. Human Reproductive Biology 51-66 (2014).

    5. Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. Endocrinology and Metabolism 973-1015 (1995).

    6. Christensen, A., Bentley, G.E., Cabrera, R., et al. Hormonal regulation of female reproduction. Horm. Metab. Res. 44(8), 587-591 (2012).

    7. Lakhani, N.J., Venitz, J., Figg, W.D., et al. Pharmacogenetics of estrogen metabolism and transport in relation to cancer. Curr. Drug Metab. 4(6), 505-513 (2003).

    Product Citations

    Chupp, D.P., Rivera, C.E., Zhou, Y., et al. A humanized mouse that mounts mature class-switched, hypermutated and neutralizing antibody responses. Nat. Immunol. 2024, 1489-1506 (2024).

    Moutard, L., Goudin, C., Jaeger, C., et al. Steroidogenesis and androgen/estrogen signaling pathways are altered in in vitro matured testicular tissues of prepubertal mice. Elife 12, RP85562 (2023).

    Taves, M.D., Donahue, K.M., Bian, J., et al. Aire drives steroid hormone biosynthesis by medullary thymic epithelial cells. Sci. Immunol. 8(86), eabo7975 (2023).

    Godiwala, P., Uliasz, T.F., Lowther, K.M., et al. Puberty suppression followed by testosterone therapy does not impair reproductive potential in female mice. Endocrinology 164(11), bqad145 (2023).

    Russo, A., Yang, Z., Heyrman, G.M., et al. Versican secreted by the ovary links ovulation and migration in fallopian tube derived serous cancer. Cancer Lett. 543, 215779 (2022).

    Kuzmanov, A., and Zwiesler-Vollick, J. A discovery-based undergraduate laboratory exercise to investigate the effect of potential endocrine disruptors on sex hormones using human cell culture. Biochem. Mol. Biol. Educ. 50(5), 527-536 (2022).

    Ashry, M., Folger, J.K., Rajput, S.K., et al. FSH stimulated bovine granulosa cell steroidogenesis involves both canonical and noncanonical WNT signaling. Domest. Anim. Endocrinol. 78, 106678 (2022).

    Estienne, A., Fréville, M., Bourdon, G., et al. Chronic dietary exposure to a glyphosate-based herbicide results in reversible increase early embryo mortality in chicken. Ecotoxicol. Environ. Saf. 241, 113741 (2022).

    Bock, S.L., Chow, M.I., Forsgren, K.L., et al. Widespread alterations to hypothalamic-pituitary-gonadal HPG) axis signaling underlie high temperature reproductive inhibition in the eurythermal sheepshead minnow (cyprinodon variegatus). Mol. Cell. Endocrinol. 525:111198, (2021).

    Zhu, Y., Hua, R., Zhou, Y., et al. Chronic exposure to mono-(2-ethylhexyl)-phthalate causes endocrine disruption and reproductive dysfunction in zebrafish. Environ. Toxicol. Chem. 35(8), 2117-2124 (2016).