A competitive ELISA for the quantification of cyclic di-AMP
Features
  • Measure cyclic di-AMP in cell lysates
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Lower limit of detection (LLOD) is 20.7 pg/ml (31.4 fmol/ml or 31.4 pM)
  • Highly specific monoclonal antibody
  • Rapid assay; get results in under 4 hours
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Cyclic di-AMP ELISA Kit

Item No. 501960

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • c-di-AMP EIA
  • Cyclic di-Adenosine monophosphate
Limit of Detection
20.7 pg/ml (31.4 pM)
Assay Range
15.6-2,000 pg/ml (23.7-3,038 pM)
Sensitivity
65.5 pg/ml (99.5 pM)
License
This product was developed in conjunction with BIOLOG Life Science Institute.
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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    96 solid wells

    $559

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    96 strip wells

    $559

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    Product Description

    Cayman’s Cyclic di-AMP ELISA Kit is a competitive assay that can be used for quantification of cyclic di-AMP in bacterial and mammalian cell lysates. The assay has a range of 15.6-2,000 pg/ml (23.7-3,038 pM) with an average sensitivity (80% B/B0) of 52 pg/ml (79 pM) and a Lower Limit of Detection (LLOD) of 21 pg/ml (32 pM). Powered by BIOLOG Life Science Institute.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Liu, Q., Tang, Z., Qian, Y., et al. Eukaryotic ADCY7 catalyzes the production of c-di-AMP to activate the NLRP3 inflammasome. Nat. Chem. Biol. 21(8), 1283-1291 (2025).

    Lee, J., and Ouellette, S.P. The second messenger signaling molecule cyclic di-AMP drives developmental cycle progression in Chlamydia trachomatis. Elife 14, RP104240 (2025).

    Priya, R., Ye, M., Raghunanadanan, S., et al. Borrelia burgdorferi c-di-AMP is a key extracellular pathogen-associated molecular pattern to elicit type I interferon responses in mammalian hosts. J. Immunol. 214(9), 2325-2337 (2025).

    Jana, B., Liu, X., Dénéréaz, J., et al. CRISPRi-TnSeq maps genome-wide interactions between essential and non-essential genes in bacteria. Nat. Microbiol. 9(9), 2395-2409 (2024).

    Kobras, C.M., Monteith, W., Somerville, S., et al. Loss of Pde1 function acts as an evolutionary gateway to penicillin resistance in Streptococcus pneumoniae. Proc. Natl. Acad. Sci. USA 120(41), e2308029120 (2023).