An inhibitor screening assay for the SARS-CoV-2 spike-ACE2 interaction
Features
  • Screen for inhibitors of spike RBD-ACE2 interaction
  • Run up to 44 samples in duplicate
  • Rapid assay; get results in <3 hours
  • Plate-based colorimetric readout (450 nm)
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SARS-CoV-2 Spike-ACE2 Interaction Inhibitor Screening Assay Kit

Item No. 502050

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Severe Acute Respiratory Syndrome Coronavirus 2 Spike-ACE2 Interaction Inhibitor Screening
Origin
Animal/Bovine, Animal/Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 solid wells

    $543

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    96 strip wells

    $543

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    Bioanalysis & Assay Development Services
    Your Vision. Our Expertise.
    • Bioanalysis and compound screening using Cayman or qualified commercial assay kits
    • Development and qualification of fit-for-purpose assays in a variety of formats and platforms
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    Product Description

    Cayman’s SARS-CoV-2 Spike-ACE2 Interaction Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of the SARS-CoV-2 spike and ACE2 interaction. The assay uses a recombinant rabbit Fc-tagged SARS-CoV-2 spike S1 RBD that binds to a plate precoated with a mouse anti-rabbit antibody. A recombinant His-tagged ACE2 protein binds the spike RBD and the complex is detected with an HRP-conjugated anti-His antibody, which is easily quantified by reading the absorbance at 450 nm. A control is included for competition of the SARS-CoV-2 spike RBD-ACE2 interaction.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Gao, B., Zhu, H., Liu, Z., et al. Chemical compositions of Scutellaria baicalensis Georgi. (Huangqin) extracts and their effects on ACE2 binding of SARS-CoV-2 spike protein, ACE2 activity, and free radicals. Int. J. Mol. Sci. 25(4), 2045 (2024).

    Gao, B., Zhu, H., Liu, Z., et al. Chemical compositions of lianqiao (Forsythia suspensa) extracts and their potential health benefits. Pharmaceuticals (Basel) 17(6), 740 (2024).

    Choe, U., Li, Y., Liu, Z., et al. Chemical compositions of dill (Anethum graveolens L.) water and ethanol extracts and their potential in blocking the SARS-CoV‑2 entry into cells and free radical scavenging capacities. ACS Food Sci. Technol. 3(10), 1654-1662 (2023).

    Li, Y., Liu, Z., Zeng, M., et al. Chemical compositions of clove (Syzygium aromaticum (L.) Merr. & L.) extracts and their potentials in suppressing SARS-CoV-2 spike protein-ACE2 binding, inhibiting ACE2, and scavenging free radicals. J. Agric. Food Chem. 70(45), 14403-14413 (2022).

    Yao, Y., Whent, M., Li, Y., et al. Chemical composition of thyme (Thymus vulgaris) extracts, potential inhibition of SARS-CoV-2 spike protein-ACE2 binding and ACE2 activity, and radical scavenging capacity. J. Agric. Food. Chem. 71(49), 19523-19530 (2023).

    Yao, Y., Choe, U., Li, Y., et al. Chemical composition of rosemary (Rosmarinus officinalis L.) extract and its inhibitory effects on SARS-CoV-2 spike protein-ACE2 interaction and ACE2 activity and free radical scavenging capacities. J. Agric. Food Chem. 71(48), 18735-18745 (2023).

    Fraternale, A., De Angelis, M., De Santis, R., et al. Targeting SARS-CoV-2 by synthetic dual-acting thiol compounds that inhibit Spike/ACE2 interaction and viral protein production. The FASEB Journal 37(2), e22741 (2023).

    Lopes, A.J.O., Calado, G.P., Fróes, Y.N., et al. Plant metabolites as SARS-CoV-2 inhibitors candidates: In silico and in vitro studies. Pharmaceuticals (Basel) 15(9), 1045 (2022).

    Cherrada, N., Elkhalifa Chemsa, A., Erol, E., et al. Phytochemical profiling of Salsola tetragona Delile by LC-HR/MS and investigation of the antioxidant, anti-inflammatory, cytotoxic, antibacterial and anti-SARS-CoV-2 activities. Saudi Pharm. J. 31(9), 101731 (2023).

    Lee, E.Y., Liu, Z., Li, Y., et al. Chemical constituents of parsley (Petroselinum crispum) leaf extract and its potential in mitigating the effects of viral infections. ACS Food Sci Technol. 3(12), 2108-2116 (2023).