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Cayman’s PAD4 Autoantibody High-Sensitivity ELISA Kit is an immunometric assay that can be used to measure IgG, IgM, or IgA isotypes of PAD4 autoantibodies in human plasma and serum without prior sample purification.
The levels of PAD4 autoantibodies in samples can be estimated using a standard curve. Please note, these levels are only approximate as polyclonal antibodies present in the sample will have different affinities to the recombinant PAD4 compared to the purified polyclonal antibody standard supplied in this kit. The standard curve spans the range of 0-125 U/ml, with a lower limit of detection (LLOD) of 0.2 U/ml. One unit of anti–PAD4 Ig is defined based on immunoreactivity and is calibrated to an internal reference standard.
Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.
WARNING This product is not for human or veterinary use.