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- Simple: only two reagent additions and one incubation step
- Rapid: results generated in 10 minutes
- Reliable: rigorous development, validation, and quality control testing
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.
Bioanalysis & Assay Development Services
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- Bioanalysis and compound screening using Cayman or qualified commercial assay kits
- Development and qualification of fit-for-purpose assays in a variety of formats and platforms
- Experienced scientists dedicated to developing the best strategy for your project
Product Description
The THUNDER™ Reader Control assay kit is a homogeneous (no-wash) time-resolved Förster resonance energy transfer (TR-FRET) assay (Figure 1). The assay workflow consists of two reagent additions (Figure 2). One binding partner is labeled with a Europium chelate donor (Europium conjugate) and the second with the far-red acceptor fluorophore (Acceptor conjugate). The binding of the two labeled partners takes place in solution and brings the two dyes into close proximity. Excitation of the donor Europium chelate molecules with a flash lamp (320 or 340 nm) or a laser (337 nm) triggers a FRET from the donor to the acceptor molecules, which in turn emit a TR-FRET signal at 665 nm. Residual energy from the Eu chelate generates light at 615 nm.
WARNING This product is not for human or veterinary use.
Contact Info
Cayman Chemical1180 East Ellsworth RoadAnn Arbor, Michigan 48108 USAToll Free: (800) 364-9897(USA and Canada only)Fax: (734) 971-3640
