A phosphorescent assay for the measurement of oxygen consumption in live cells or mitochondria
Features
  • Real-time kinetic OCR monitoring on any standard fluorescent plate reader
  • Includes glucose oxidase as a reference for oxygen depletion
  • Works with live cells and isolated mitochondria
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Extracellular Oxygen Consumption Assay Kit

Item No. 502880

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • OCR
Origin
Fungus/Aspergillus niger, Synthetic
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea

    $495

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    Product Description

    Cayman’s Extracellular Oxygen Consumption Assay Kit uses a phosphorescent oxygen-sensitive probe. Because of its inability to cross the membrane, the probe selectively reports on changes in oxygen levels in the medium. The probe’s signal is quenched upon binding to oxygen, resulting in a signal that is inversely proportional to the amount of dissolved oxygen present in culture medium. As mitochondria consume oxygen during respiration, the extracellular oxygen concentration decreases, leading to an increase in signal intensity over time. This assay is compatible with standard fluorescence plate readers and provides a simple and sensitive method for determining OCR in living cells and isolated mitochondria.1

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hynes, J., Natoli, E., Jr., and Will, Y. Fluorescent pH and oxygen probes of the assessment of mitochondrial toxicity in isolated mitochondria and whole cells. Curr. Protoc. Toxicol. 2.16.11-12.16.22 (2009).