Stem Cell Expansion Reagent Kit (SCB)

The components of this kit can be combined to produce the small molecule cocktail SCB. When mixed with the appropriate cell culture media, SCB promotes cell expansion and increases the clonogenic potential of genetically stable hematopoietic stem cells (HSCs).^1,2 SB-431542 is a potent and selective inhibitor of activin-like kinase 5 (ALK5), also known as TGF-β receptor type 1 (TGF-βRI), that increases the proliferation of HSCs.³ CHIR99021 is an inhibitor of glycogen synthase kinase 3 (GSK-3) that regulates Wnt/β-catenin signaling and plays an important role in the differentiation of HSCs.⁴ bpV(pic) is a selective PTEN inhibitor that increases HSC proliferation and survival.⁵

Each component in this kit is provided separately, and instructions are included to reconstitute and mix these reagents for direct dilution into cell culture media. The kit is intended to supplement up to 2 L of culture media. Some reagents may be provided in excess and may be properly disposed of after cell media preparation. Prior to use, reagents must be stored as indicated at -20°C.

WARNING This product is not for human or veterinary use.

1. Zarrabi, M., Afzal, E., and Ebrahimi, M. P116-A chemical approach to ex vivo expansion of cord blood hematopoietic stem cells. International Congress on Stem Cells and Regenerative Medicine (2015).

2. Zarrabi, M., Afzal, E., Asghari, M.H., et al. Combination of SB431542, chir9901, and bpV as a novel supplement in the culture of umbilical cord blood hematopoietic stem cells. Stem Cell Res. Ther. 11(1), 474 (2020).

3. Csaszar, E., Kirouac, D.C., Yu, M., et al. Rapid expansion of human hematopoietic stem cells by automated control of inhibitory feedback signaling. Cell Stem Cell 10, 218-229 (2012).

4. Huang, J., Nguyen-McCarty, M., Hexner, E.O., et al. Maintenance of hematopoietic stem cells through regulation of Wnt and mTOR pathways. Nat. Med. 18(12), 1778-1785 (2012).

5. Zhu, A.Z., Ma, Z., Wolff, E.V., et al. HES1 is required for mouse fetal hematopoiesis. Stem Cell Res. Ther. 15, 235 (2024).