A thermal shift assay for the detection of analyte binding to HSA
Features
  • Simple 2 hour assay allows for evaluation of serum albumin binding at any site
  • Small amounts of analyte needed, suitable for early-stage drug development
  • Performed using an RT-PCR device, no radiolabeled compound or spin columns needed
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

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Human Serum Albumin Binding Assay Kit

Item No. 502941

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • HSA Binding
Origin
Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    1 ea

    $250

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    Product Description

    Cayman’s HSA Binding Assay Kit provides a fluorometric thermal shift assay (TSA) method for measuring analyte binding to HSA. Probe fluorescence can be quantified at excitation and emission wavelengths of 468 and 507 nm, respectively, or the SYBR® Green channel of a qPCR instrument. This method detects increases in protein stabilization that occur with compound binding via measurement of the protein melting temperature (Tm). The probe used in this assay does not affect the stability of HSA, resulting in very consistent melting profiles and drug-induced thermal shifts. This assay is validated for evaluation of compound binding to well-characterized drug-binding sites, such as Sudlow’s Sites I and II.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.