A competitive assay that can be used for quantification of PGD2
Features
  • Measure PGD2 in multiple sample types
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure PGD2 levels down to 350 pg/ml
  • Rapid assay; get results in under 4 hours
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Prostaglandin D2 Express ELISA Kit

Item No. 512041

Technical Information
Synonyms
  • PGD2 Express EIA Kit
Assay Range
117.2-15,000 pg/ml
Sensitivity
80% B/B0: 350 pg/ml
Cross Reactivity
(Prostaglandin D2) 100%(Prostaglandin F) 92.4%(Prostaglandin J2) 21.6%(Prostaglandin E2) 2.86%(Thromboxane B2) 2.54%(11β-Prostaglandin F) 1.99%(8-iso Prostaglandin F) 1.90%(Prostaglandin A2) 0.72%(12(S)-HHTrE) 0.16%(6-keto Prostaglandin F) 0.05%(13,14-dihydro-15-keto Prostaglandin D2) 0.02%(Arachidonic Acid) <0.01%(Leukotriene D4) <0.01%(tetranor-PGDM) <0.01%(tetranor-PGEM) <0.01%(tetranor-PGFM) <0.01%(tetranor-PGJM) <0.01%
Origin
Animal/Bovine, Animal/Eel, Animal/Goat, Animal/Mouse
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    Product Description

    Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kDa glutathione-independent lipocalin-type PGD2 synthase. This PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase. This PGD2 which is formed in the intracellular and vascular compartments is rapidly metabolized to 11β-PGF and other metabolites. Measurement of the parent eicosanoid PGD2 is appropriate in cell culture lysates and in CSF, where concentrations of several hundred pg/ml have been measured. Cayman’s PGD2 Express ELISA Kit is a competitive assay that can be used for quantification of PGD2 in cell culture lysates and enzymatic reactions. This ELISA offers the convenience of a fast assay with a two hour incubation time and one hour development time, while still achieving an assay range of approximately 117.2-15,000 pg/ml and a sensitvity (80% B/B0) of approximately 350 pg/ml.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.