Competitive ELISA for quantitation of a primary metabolite of PGE2 in biological samples
Features
  • Converts major urinary and plasma metabolites of PGE2 to a single, stable derivative that is easily quantified
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure PGEM levels down to 2 pg/ml
  • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
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Prostaglandin E Metabolite ELISA Kit

Item No. 514531

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • PGEM
  • PGEM EIA
  • PGE Metabolite
  • PGE Metabolite EIA
Assay Range
0.39-50 pg/ml
Sensitivity
80% B/B0: 2 pg/ml
Cross Reactivity
(13,14-dihydro-15-keto Prostaglandin E1 (derivatized)) 100%(13,14-dihydro-15-keto Prostaglandin E2 (derivatized)) 100%(Bicyclo Prostaglandin E2) 100%(13,14-dihydro-15-keto PGA2) 100%(Bicyclo Prostaglandin E1) 38%(13,14-dihydro-15-keto Prostaglandin D2 (derivatized)) 0.08%(13,14-dihydro-15-keto Prostaglandin F2α (derivatized)) 0.02%(Arachidonic Acid) <0.01%(Leukotriene B4) <0.01%(tetranor-PGEM) <0.01%(tetranor-PGFM) <0.01%(Prostaglandin D2) <0.01%(Prostaglandin E1) <0.01%(6-keto Prostaglandin E1) <0.01%(Prostaglandin E2) <0.01%(Prostaglandin F1α) <0.01%(6-keto Prostaglandin F1α) <0.01%(Prostaglandin F2α) <0.01%(Thromboxane B2) <0.01%
Origin
Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Cayman’s Prostaglandin E Metabolite (PGEM) assay converts downstream metabolites of PGE2, such as 13,14-dihydro-15-keto PGE2 and 13,14-dihydro-15-keto PGA2, to stable derivatives that can be easily quantified in a competitive ELISA format. This assay is the method of choice for analysis of samples, such as plasma, serum and urine, in which PGE2 has undergone extensive metabolism prior to collection. The assay has a range from 0.39-50 pg/ml and an average sensitivity (80% B/B0) of 2 pg/ml.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Fenske, R.J., Weeks, A.M., Daniels, M., et al. Plasma prostaglandin E2 metabolite levels predict type 2 diabetes status and one-year therapeutic response independent of clinical markers of inflammation. Metabolites 12(12), 1234 (2022).

    Werner, S., Mesch, S., Hillig, R.C., et al. Discovery and characterization of the potent and selective P2X4 inhibitor N-[4-(3-chlorophenoxy)-3-sulfamoylphenyl]-2-phenylacetamide (BAY-1797) and structure-guided amelioration of Its CYP3A4 induction profile. J. Med. Chem. 62(24), 11194-11217 (2019).

    Saldaña, L., Bensiamar, F., Vallés, G., et al. Immunoregulatory potential of mesenchymal stem cells following activation by macrophage-derived soluble factors. Stem Cell Res. Ther. 10(1), 58 (2019).

    Schmidleithner, L., Thabet, Y., Schönfeld, E., et al. Enzymatic activity of HPGD in Treg cells suppresses Tconv cells to maintain adipose tissue homeostasis and prevent metabolic dysfunction. Immunity 50(5), 1232-1248 (2019).

    Wang, H., Sethi, G., Loke, W.-K., et al. Des-aspartate-angiotensin I attenuates mortality of mice exposed to gamma radiation via a novel mechanism of action. PLoS One 10(9), e0138009 (2015).

    Apostoli, A.J., Skelhorne-Gross, G.E., Rubino, R.E., et al. Loss of PPARcexpression in mammary secretory epithelial cellscreates a pro-breast tumorigenic environment. Int. J. Cancer 134(5), 1055-1066 (2014).

    Richardson, G.F., McNiven, M.A., Petit, H.V., et al. The effects of dietary omega fatty acids on pregnancy rate, plasma prostaglandin metabolite levels, serum progesterone levels, and milk fatty-acid profile in beef cows. Can. J. Vet. Res. 77(4), 314-318 (2013).

    Banerjee, D., Bauri, A.K., Guha, R.K., et al. Healing properties of malabaricone B and malabaricone C, against indomethacin-induced gastric uleration and mechanism of action. Eur. J. Pharm. 578(2-3), 300-312 (2008).

    Petit, H.V., Small, J.A., Palin, M.F., et al. Effects of flaxseed supplementation on endometrial expression of ISG17 and intrauterine prostaglandin concentrations in primiparous dairy cows submitted to GnRH-based synchronized ovulation. Can. J. Animal Sci. 87(3), 343-352 (2007).

    Sun, R., Miller, R.L., Hemmert, A.C., et al. Chronic dDAVP infusion in rats decreases the expression of P2Y2 receptor in inner medulla and P2Y2 receptor-mediated PGE2 release by IMCD. Am. J. Physiol. Renal Physiol. 289(4), F768-776 (2005).