6-keto Prostaglandin F1α ELISA Kit | Cayman Chemical
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6-keto Prostaglandin F ELISA Kit

Item № 515211
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 solid wells $270.00 0.00
     96 strip wells $270.00 0.00
     480 solid wells $1,013.00 0.00
     480 strip wells $1,013.00 0.00

Pricing updated 2018-05-27. Prices are subject to change without notice.

Description
Features
  • Detect 6-keto PGF, a surrogate for prostacyclin synthesis in multiple sample types
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 6-keto PGF levels down to 6 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • 6-keto PGF EIA Kit

Prostacyclin (Prostaglandin I2; PGI2) is formed from arachidonic acid primarily by the vascular endothelium and renal cortex.1,2 It is a potent vasodilator and inhibitor of platelet aggregation.2 PGI2 is non-enzymatically hydrated to 6-keto PGF (t½ = 2-3 minutes), and then quickly converted to the major metabolite, 2,3-dinor-6-keto PGF (t½ = 30 minutes).3,4,5 Prostacyclin was once thought to be a circulating hormone that regulated platelet-vasculature interactions, but the rate of secretion into circulation coupled with the short half-life indicate that prostacyclin functions locally.6 Although 6-keto PGF is commonly measured in plasma and urine as an estimate of prostacyclin synthesis, it should be noted that there may be more than one source of PGI2 in these samples. For instance, venipuncture may cause the release of prostacyclin which will artifactually increase the 6-keto PGF concentration in plasma.7 Urinary concentrations of 6-keto PGF are confounded by the fact that some plasma prostacyclin (~14%) is excreted into urine as 6-keto PGF and the remainder is of renal origin.6,7 Therefore, it is important to take these factors into account when analyzing data.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • 6-keto PGF EIA Kit
Assay Range
1.6-1,000 pg/ml
Sensitivity
80% B/B0: 6 pg/ml
Mid-point
50% B/B0: 20-60 pg/ml
Cross Reactivity
  • (6-keto Prostaglandin F) 100%
  • (6-keto Prostaglandin E1) 33.9%
  • (Prostaglandin F) 28%
Show all 12

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Moncada, S., Gryglewski, R., Bunting, S., et al. An enzyme isolated from arteries transforms prostaglandin endoperoxides to an unstable substance that inhibits platelet aggregation. Nature 263, 663-665 (1976).

2. Navamani, M., Morgan, M., and Williams, R.J. Ethanol modulates N-methyl-D-aspartate-evoked arachidonic acid release from neurones. European Journal of Pharmacology 340, 27-34 (1997).

3. Samuelsson, B., Goldyne, M., Granström, E., et al. Prostaglandins and thromboxanes. Annu. Rev. Biochem. 47, 997-1029 (1978).

4. Dusting, G.J., Moncada, S., and Vane, J.R. Prostacyclin (PGX) is the endogenous metabolite responsible for relaxation of coronary arteries induced by arachidonic acid. Prostaglandins 13, 3-15 (1977).

5. Rosenkranz, B., Fischer, C., Reimann, I., et al. Identification of the major metabolite of prostacyclin and 6-ketoprostaglandin F in man. Biochimica et Biophysica Acta 619, 207-213 (1980).

6. Frolich, J.C. Measurement of eicosanoids. Prostaglandins 27, 349-369 (1984).

7. Catella, F., Nowak, J., and Fitzgerald, G.A. Measurement of renal and non-renal eicosanoid synthesis. American Journal of Medicine 81, 23-29 (1986).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for 6-keto Prostaglandin F ELISA Kit

View all analysis workbooks

View the pre-configured analysis tool for 6-keto Prostaglandin F ELISA Kit on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of 6-keto Prostaglandin F ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640