Prostaglandin F_2α ELISA Kit

Prostaglandin F_2α (PGF_2α) is one of the five primary PGs derived enzymatically directly from the endoperoxide PGH₂. It was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF_2α is a potent luteolytic agent and is used to induce ovulation in domestic livestock. It is also a potent uterine stimulant, and is part of the cascade of myometrial stimulants which induce and sustain labor. PGF_2α is rapidly metabolized to 13,14-dihydro-15-keto PGF_2α in vivo, and the levels of this metabolite in both plasma and amniotic fluid are elevated during active labor in several different mammalian species. Like all of the primary PGs, PGF_2α. has a very short half-life in the general circulation. The plasma concentration of PGF_2α in humans is <10 pg/ml, and probably no more than 1-2 pg/ml.¹ PGF_2α is rapidly metabolized by 15-hydroxy PG dehydrogenase and both β- and ω-oxidation systems to a variety of polar metabolites.² In primary cultures, the metabolism of PGF_2α is much more limited, and it may collect in the supernatant medium to easily measured concentrations.³ Amnionic and placental tissue PGF_2α content in the range from 100-250 ng/g of tissue has been reported.⁴ However, this study did not address the presence of isoprostanes and reported measurements using an unvalidated RIA method. A controlled GC- or LC-MS study of tissue PG content has not been reported. Tissue PG measurements in general are difficult to perform, vary widely, and are challenging to interpret. Cayman also offers 13,14-dihydro-15-keto Prostaglandin F_2α ELISA Kit (Item No. 516671) for quantification of 13,14-dihydro-15-keto Prostaglandin F_2α in plasma and other sample matrices.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

1. Schweer, H., Kammer, J., and Seyberth, H.W. Simultaneous determination of prostanoids in plasma by gas chromatography-negative-ion chemical ionization mass spectrometry. J. Chromatogr. 338(2), 273-280 (1985).

2. Granström, E., and Kindahl, H. Species differences in circulating prostaglandin metabolites relevance for the assay of prostaglandin release. Biochim. Biophys. Acta 713, 555-569 (1982).

3. Ritzi, E.M., Boto, W.O., and Stylos, W.A. Measurement of initial prostaglandin F metabolites in medium of BALB/c 3T3 and SV3T3 mouse fibroblast cultures. Biochem. Biophys. Res. Commun. 63(1), 179-186 (1975).

4. Reece, M.S., McGregor, J.A., Allen, K.G.D., et al. Prostaglandins in selected reproductive tissues in preterm and full term gestations. Prostaglandins Leukot. Essent. Fatty Acids 55, 303-307 (1996).

Lupicka, M., Socha, B., Szczepánska, A., et al. Expression of pluripotency markers in the bovine uterus with adenomyosis. Reprod. Biol. Endocrinol. 13, 110 (2015).