8-Isoprostane ELISA Kit | Cayman Chemical
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8-Isoprostane ELISA Kit

Item № 516351
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 solid wells $270.00 0.00
     96 strip wells $270.00 0.00
     480 solid wells $1,013.00 0.00
     480 strip wells $1,013.00 0.00

Pricing updated 2018-06-23. Prices are subject to change without notice.

Description
Features
  • Measure 8-isoprostane, a biomarker of oxidative stress and antioxidant deficiency
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 8-isoprostane levels down to 3 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • iPF-III
  • 8-Isoprostane EIA Kit
  • 8-epi Prostaglandin F
  • 8-iso Prostaglandin F

The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also appear in the plasma and urine under normal conditions and are elevated by oxidative stress. At least one of the isoprostanes, 8-isoprostane (8-epi PGF), has been shown to have biological activity. It is a potent pulmonary and renal vasoconstrictor1 and has been implicated as a causative mediator of hepatorenal syndrome and pulmonary oxygen toxicity.2 8-Isoprostane has been proposed as a marker of antioxidant deficiency and oxidative stress and elevated levels have been found in heavy smokers.3 8-Isoprostane levels are also a relative indicator of sample integrity for lipid-containing samples such as serum, plasma, and whole cell preparations.4 Plasma from healthy volunteers contains modest amounts of 8-isoprostane (40-100 pg/ml) that increase with the age of the test subject.5 Normal human urinary levels range from 10-50 ng/mmol creatinine, which is an order of magnitude higher than many enzymatically derived eicosanoids.5,6

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • iPF-III
  • 8-Isoprostane EIA Kit
  • 8-epi Prostaglandin F
  • 8-iso Prostaglandin F
Assay Range
0.8-500 pg/ml
Sensitivity
80% B/B0: 3 pg/ml
Mid-point
50% B/B0: 7-20 pg/ml
Cross Reactivity
  • (8-Isoprostane) 100%
  • (8-iso Prostaglandin F ethanolamide) 100%
  • (8-iso Prostaglandin F) 20.6%
Show all 26

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Banerjee, M., Kang, K.H., Morrow, J.D., et al. Effects of a novel prostaglandin, 8-epi-PGF, in rabbit lung in situ. American Journal of Physiology 263, H660-H663 (1992).

2. Vacchiano, C.A., and Tempel, G.E. Role of nonenzymatically generated prostanoid, 8-iso-PGF, in pulmonary oxygen toxicity. Journal of Applied Physiology 77, 2912-2917 (1994).

3. Morrow, J.D., Frei, B., Longmire, A.W., et al. Increase in circulating products of lipid peroxidation (F2-isoprostanes) in smokers. New England Journal of Medicine 332, 1198-1203 (1995).

4. Morrow, J.D., Hill, K.E., Burk, R.F., et al. A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cyclooxygenase, free radical-catalyzed mechanism. Proceedings of the National Academy of Sciences of the United States of America 87, 9383-9387 (1990).

5. Wang, Z., Ciabattoni, G., Créminon, C., et al. Immunological characterization of urinary 8-epi-prostaglandin F excretion in man. Journal of Pharmacology and Experimental Therapeutics 275, 94-100 (1995).

6. Reilly, M.P., Barry, P., Lawson, J.A., et al. Urinary 8-epi PGF: An index of oxidant stress in vivo. Fibrinolysis & Proteolysis 11, 81-84 (1997).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for 8-Isoprostane ELISA Kit

View all analysis workbooks

View the pre-configured analysis tool for 8-Isoprostane ELISA Kit on MyAssays.com

FAQ

What is the main difference between 8-Isoprostane EIA Kit and 8-Isoprostane Express EIA Kit?

Between the two 8-Isoprostane EIA kits you mention, 8-Isoprostane EIA Kit requires an overnight incubation and has a detection limit of 2.7 pg/ml. The 8-Isoprostane Express EIA Kit, can be performed in one day but has a detection limit of 10 pg/ml.

In the 8-isoprostane EIA Kit, what amount of fresh homogenized tissue is needed?

There is no fixed amount of tissue required for the assay, as the amount of tissue needed will vary depending on the experimental conditions. Sample purification for tissues is recommended as described in the kit booklet.

Are 8-Isoprostane and 8-iso-Prostalgandin F2α the same thing?

Yes, 8-Isoprostane and 8-iso-Prostaglandin F2α are synonyms.

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You may be eligible to receive a free sample of 8-Isoprostane ELISA Kit under the Cayman Challenge program.

Cayman Chemical

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Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

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