A competitive ELISA for the quantification of 8-isoprostane
Features
  • Measure 8-isoprostane, a biomarker of oxidative stress and antioxidant deficiency
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 8-isoprostane levels down to 3 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
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8-Isoprostane ELISA Kit

Item No. 516351

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • iPF2α-III
  • 8-Isoprostane EIA Kit
  • 8-epi Prostaglandin F2α
  • 8-iso Prostaglandin F2α
Assay Range
0.8-500 pg/ml
Sensitivity
3 pg/ml
Cross Reactivity
(8-Isoprostane) 100%(8-iso Prostaglandin F2α ethanolamide) 100%(8-iso Prostaglandin F3α) 20.6%(2,3-dinor-8-iso Prostaglandin F2α) 4.00%(8-iso Prostaglandin E2) 1.84%(2,3-dinor-8-iso Prostaglandin F1α) 1.7%(8-iso Prostaglandin E1) 1.56%(Prostaglandin F1α) 0.71%(Prostaglandin F3α) 0.66%(Prostaglandin E1) 0.39%(Prostaglandin D2) 0.16%(6-keto Prostaglandin F1α) 0.14%(Prostaglandin F2α) 0.14%(2,3-dinor-6-keto Prostaglandin F1α) 0.09%(8-iso Prostaglandin F1β) 0.08%(Thromboxane B2) 0.08%(11-dehydro Thromboxane B2) 0.07%(11β-Prostaglandin F2α) 0.03%(Prostaglandin E2) 0.02%(8-iso-15(R)-Prostaglandin F2α) 0.02%(8,12-epi iPF2α-III) <0.01%(iPF2α-VI) <0.01%(8,12-epi iPF2α-VI) <0.01%(tetranor-PGEM) <0.01%(tetranor-PGFM) <0.01%(13,14-dihydro-15-keto Prostaglandin F2α) <0.01%
Origin
Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Cayman’s 8-Isoprostane ELISA Kit is a competitive assay that can be used for quantification of 8-isoprostane in plasma, urine, and other sample matrices. The assay has a range from 0.8-500 pg/ml and a sensitivity (80% B/B0) of approximately 3 pg/ml.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Dinesan, A.S., and Ravindran, R. Evaluation of salivary 8-isoprostane in oral lichen planus: Case control study. J. Orofac. Sci. 12, 113-118 (2021).

    Vengerfeldt, V., Mändar, R., Saag, M., et al. Oxidative stress in patients with endodontic pathologies. J. Pain Res. 10, 2031-2040 (2017).

    Karina, M.J., Myslinska, D., Dzik, K.P., et al. The electrical stimulation of the bed nucleus of the stria terminalis causes oxidative stress in skeletal muscle of rats. Oxid. Med. Cell. Longev. 4671213, (2018).

    Salvi, A., Patki, G., Khan, E., et al. Relationship between advanced glycation end products and increased lipid peroxidation in semen of diabetic men. Oxid. Med. Cell. Longev. 5059043 (2016).

    Karthivashan, G., Arulselvan, P., Alimon, A.R., et al. Competing role of bioactive constituents in Moringa oleifera extract and conventional nutrition feed on the performance of Cobb 500 broilers. Biomed Res. Int. 970398, (2015).

    Churg, A., Marshall, C.V., Sin, D.D., et al. Late intervention with a myeloperoxidase inhibitor stops progression of experimental chronic obstructive pulmonary disease. Am. J. Respir. Crit. Care Med. 185(1), (2012).

    Aldini, G., Orioli, M., Rossoni, G., et al. The carbonyl scavenger carnosine ameliorates dyslipidaemia and renal function in Zucker obese rats. J. Cell. Mol. Med. 15(6), 1339-1354 (2011).

    Campo, G.M., Avenoso, A., Campo, S., et al. Chondroitin-4-sulphate inhibits NF-kB translocation and caspase activation in collagen-induced arthritis in mice. Osteoarthritis Cartilage 16(12), 1474-1483.

    Bełtowski, J., Wójcicka, G., and Jamroz, A. Leptin decreases plasma paraoxonase 1 (PON1) activity and induces oxidative stress: The possible novel mechanism for proatherogenic effect of chronic hyperleptinemia. Atherosclerosis 170(1), 21-29 (2003).