8-Isoprostane Express ELISA Kit | Cayman Chemical
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8-Isoprostane Express ELISA Kit

Item № 516360
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 solid wells $285.00 0.00
     96 strip wells $285.00 0.00
     480 solid wells $1,069.00 0.00
     480 strip wells $1,069.00 0.00

Pricing updated 2018-11-14. Prices are subject to change without notice.

Description
Features
  • Measure 8-isoprostane, a biomarker of oxidative stress and antioxidant deficiency
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 8-isoprostane levels down to 10 pg/ml
  • Rapid assay; get results in under 4 hours
Synonyms
  • iPF-III
  • 8-Isoprostane Express EIA Kit
  • 8-epi Prostaglandin F
  • 8-iso Prostaglandin F

The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also appear in the plasma and urine under normal conditions and are elevated by oxidative stress. At least one of the isoprostanes, 8-isoprostane (8-epi PGF), has been shown to have biological activity. It is a potent pulmonary and renal vasoconstrictor1 and has been implicated as a causative mediator of hepatorenal syndrome and pulmonary oxygen toxicity.2 8-Isoprostane has been proposed as a marker of antioxidant deficiency and oxidative stress and elevated levels have been found in heavy smokers.3 8-Isoprostane levels are also a relative indicator of sample integrity for lipid-containing samples such as serum, plasma, and whole cell preparations.4 Plasma from healthy volunteers contains modest amounts of 8-isoprostane (40-100 pg/ml) that increase with the age of the test subject.5 Normal human urinary levels range from 10-50 ng/mmol creatinine, which is an order of magnitude higher than many enzymatically derived eicosanoids.5,6 Cayman’s 8-Isoprostane Express ELISA is a competitive assay that can be used for quantification of 8-isoprostane in plasma, serum, urine, whole blood, tissue culture supernatants, and other sample matrices.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • iPF-III
  • 8-Isoprostane Express EIA Kit
  • 8-epi Prostaglandin F
  • 8-iso Prostaglandin F
Assay Range
2.5-1,500 pg/ml
Sensitivity
80% B/B0: 10 pg/ml
Mid-point
50% B/B0: 40-60 pg/ml
Cross Reactivity
  • (8,12-epi iPF-VI) <0.01%
  • (tetranor-PGEM) <0.01%
  • (tetranor-PGFM) <0.01%
Show all 26

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Banerjee, M., Kang, K.H., Morrow, J.D., et al. Effects of a novel prostaglandin, 8-epi-PGF, in rabbit lung in situ American Journal of Physiology 263, H660-H663 (1992).

2. Vacchiano, C.A., and Tempel, G.E. Role of nonenzymatically generated prostanoid, 8-iso-PGF, in pulmonary oxygen toxicity Journal of Applied Physiology 77, 2912-2917 (1994).

3. Morrow, J.D., Frei, B., Longmire, A.W., et al. Increase in circulating products of lipid peroxidation (F2-isoprostanes) in smokers New England Journal of Medicine 332, 1198-1203 (1995).

4. Morrow, J.D., Hill, K.E., Burk, R.F., et al. A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cyclooxygenase, free radical-catalyzed mechanism Proceedings of the National Academy of Sciences of the United States of America 87, 9383-9387 (1990).

5. Wang, Z., Ciabattoni, G., Créminon, C., et al. Immunological characterization of urinary 8-epi-prostaglandin F excretion in man Journal of Pharmacology and Experimental Therapeutics 275, 94-100 (1995).

6. Reilly, M.P., Barry, P., Lawson, J.A., et al. Urinary 8-epi PGF: An index of oxidant stress in vivo Fibrinolysis & Proteolysis 11, 81-84 (1997).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for 8-Isoprostane Express ELISA Kit

View all analysis workbooks

View the pre-configured analysis tool for 8-Isoprostane Express ELISA Kit on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of 8-Isoprostane Express ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640