For the measurement of 13,14-dihydro-15-keto PGF2α in plasma
Features
  • Detect 13,14-dihydro-15-keto PGF2α, a stable metabolite of PGF2α
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 13,14-dihydro-15-keto PGF2α levels down to 16.3 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
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13,14-dihydro-15-keto Prostaglandin F2α ELISA Kit

Item No. 516671

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 13,14-dihydro-15-keto PGF2α EIA Kit
  • PGFM EIA Kit
Assay Range
2.3-5,000 pg/ml
Sensitivity
80% B/B0: 15 pg/ml
Cross Reactivity
(13,14-dihydro-15-keto Prostaglandin F2α) 100%(13,14-dihydro-15-keto Prostaglandin E2) 2.7%(15-keto Prostaglandin F2α) 1.8%(Prostaglandin D2) <0.01%(Prostaglandin E2) <0.01%(Prostaglandin F1α) <0.01%(2,3-dinor-6-keto Prostaglandin F1α) <0.01%(6-keto Prostaglandin F1α) <0.01%(Prostaglandin F2α) <0.01%(8-iso Prostaglandin F2α) <0.01%(8-iso-13,14-dihydro-15-keto Prostaglandin F2α) <0.01%(8-iso-15-keto Prostaglandin F2α) <0.01%(Prostaglandin F2β) <0.01%(8-iso Prostaglandin F3α) <0.01%(Thromboxane B2) <0.01%
Origin
Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Prostaglandin F2α (PGF2α) is one of the five primary prostaglandins derived enzymatically directly from the endoperoxide PGH2. PGF2α was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF2α is a potent luteolytic agent and is used to induce ovulation in domestic livestock. It is also a potent uterine stimulant, and is part of the cascade of myometrial stimulants which induce and sustain labor. PGF2α is rapidly metabolized to 13,14-dihydro-15-keto PGF2α in vivo, by the enzymes 15-hydroxy prostaglandin dehydrogenase and Δ13-reductucase.1,2,3,4 Measurement of 13,14-dihydro-15-keto PGF2α in plasma can be used as a marker of the in vivo production of PGF2α.5,6 The Cayman Chemical 13,14-dihydro-15-keto PGF2α Enzyme Immunoassay is a competitive assay that provides measurements of 13,14-dihydro-15-keto PGF2α within the range of 2.3-5,000 pg/ml, typically with a detection limit (80% B/B0) of approximately 15 pg/ml. Although the tracer and antiserum for this assay have been sold separately for many years, the reagents are now combined with an accurate standard and packaged together in a convenient assay kit. The assay has been validated for the measurement of 13,14-dihydro-15-keto PGF2α in plasma, a common matrix for measurement of this PGF2α metabolite.5

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Samuelsson, B., Granström, E., Green, K., et al. Prostaglandins. Annu. Rev. Biochem. 44, 669-695 (1975).

    2. Keirse, M.J.N.C., and Turnbull, A.C. Metabolism of prostaglandins within the pregnant uterus. Br. J. Obstet. Gynaecol. 82, 887-893 (1975).

    3. Basu, S., and Stjernschantz, J. Δ13-reductase dependent metabolism of prostaglandins in the mammalian brain and eye. Prostaglandins Leukot. Essent. Fatty Acids 57(3), 305-310 (1997).

    4. Ensor, C.M., Yang, J.Y., Okita, R.T., et al. Cloning and sequence analysis of the cDNA for human placental NAD+-dependent 15-hydroxyprostaglandin dehydrogenase. The Journal of Biological Chemisty 265, 14888-14891 (1990).

    5. Del Vecchio, R.P., Maxey, K.M., and Lewis, G.S. A quantitative solid-phase enzymeimmunoassay for 13,14-dihydro-15-keto-prostaglandin F2α in plasma. Prostaglandins 43(4), 321-330 (1992).

    6. Meyer, H.H.D., Eisele, K., and Osaso, J. A biotin-streptavidin amplified enzyme immunoassay for 13,14-dihydro-15-keto-PGF2α. Prostaglandins 38(3), 375-383 (1989).

    Product Citations

    Richardson, G.F., McNiven, M.A., Petit, H.V., et al. The effects of dietary omega fatty acids on pregnancy rate, plasma prostaglandin metabolite levels, serum progesterone levels, and milk fatty-acid profile in beef cows. Can. J. Vet. Res. 77(4), 314-318 (2013).

    Farhat, A., Philibert, P., Sultan, C., et al. Hematopoietic-Prostaglandin D2 synthase through PGD2 production is involved in the adult ovarian physiology. J. Ovarian Res. 4, 3 (2011).