A sensitive detection method for measuring the free acid form of fluprostenol
Features
  • Measure the free acid form of fluprostenol
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure fluprostenol levels down to 16 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
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Fluprostenol ELISA Kit

Item No. 516761

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 16-m-trifluoromethylphenoxy tetranor Prostaglandin F2α EIA Kit
Assay Range
7.8-1,000 pg/ml
Sensitivity
80% B/B0: ~16 pg/ml
Cross Reactivity
(Fluprostenol) 100%(2,3-dinor Fluprostenol) 100%(Fluprostenol lactone diol) 100%(9-keto Fluprostenol) 83%(15(S)-Fluprostenol isopropyl ester) 27%(11-keto Fluprostenol) 15.2%(Fluprostenol isopropyl ester) 10%(9-keto Fluprostenol isopropyl ester) 8.5%(15-keto Fluprostenol free acid) 0.9%(15-keto Fluprostenol isopropyl ester) 0.02%(Latanoprost) <0.01%(Latanoprost (free acid)) <0.01%(15-epi Latanoprost) <0.01%(5,6-trans Latanoprost) <0.01%(tetranor-PGEM) <0.01%(tetranor-PGFM) <0.01%(Prostaglandin D2) <0.01%(Prostaglandin E2) <0.01%(Prostaglandin E2 Ethanolamide) <0.01%(6-keto Prostaglandin F1α) <0.01%(Prostaglandin F2α) <0.01%(Prostaglandin F2α Ethanolamide) <0.01%(5-trans Prostaglandin F2α) <0.01%(17-phenyl trinor Prostaglandin F2α) <0.01%(17-phenyl trinor Prostaglandin F2α ethyl amide) <0.01%(Thromboxane B2) <0.01%(Unoprostone) <0.01%(Unoprostone isopropyl ester) <0.01%
Origin
Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    96 solid wells

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    Product Description

    Fluprostenol is a metabolically stable analog of PGF2α with potent FP receptor agonist activity.1,2 The isopropyl ester of fluprostenol has recently been approved for use as an ocular hypotensive drug for the treatment of glaucoma and is sold under the Alcon trade name Travoprost.3,4 Acting as a prodrug, Fluprostenol isopropyl ester is converted by esterase activity in the cornea to yield the corresponding active free acid, (+)-fluprostenol.5 Fluprostenol is also used in the veterinary field for the induction of estrus in livestock. Cayman’s Fluprostenol ELISA is a sensitive detection method for measuring the free acid form of fluprostenol. The assay also detects the isopropyl ester form of fluprostenol, but with less sensitivity. Samples containing a mixture of the isopropyl ester and free acid should be purified and the compounds separated prior to analysis. The assay has been validated for use in aqueous humor samples.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Abramovitz, M., Boie, Y., Nguyen, T., et al. Cloning and expression of a cDNA for the human prostanoid FP receptor. The Journal of Biological Chemisty 269(4), 2632-2636 (1994).

    2. Lake, S., Gullberg, H., Wahlqvist, J., et al. Cloning of the rat and human prostaglandin F2α receptors and the expression of the rat prostaglandin F2α receptor. FEBS Lett. 355(3), 317-325 (1994).

    3. Sorbera, L.A., and Castañer, J. Travoprost. Drugs Future 25(1), 41-45 (2000).

    4. Hellberg, M.R., Sallee, V.L., McLaughlin, M.A., et al. Preclinical efficacy of travoprost, a potent and selective FP prostaglandin receptor agonist. J. Ocul. Pharmacol. Ther. 17(5), 421-432 (2001).

    5. Stjernschantz, J., and Resul, B. Phenyl substituted prostaglandin analogs for glaucoma treatment. Drugs Future 17(8), 691-704 (1992).