A sensitive detection method for measuring both the free acid and ethyl amide forms of 17-phenyl trinor PGF2α
Features
  • Measure 17-phenyl trinor PGF2α (also known as bimatoprost)
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 17-phenyl trinor PGF2α levels down to 2.6 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
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17-phenyl trinor Prostaglandin F2α ELISA Kit

Item No. 516821

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Bimatoprost (free acid)
  • 17-phenyl trinor Prostaglandin F2α EIA Kit
Assay Range
1.56-200 pg/ml
Sensitivity
80% B/B0: 2.6 pg/ml
Cross Reactivity
(17-phenyl trinor Prostaglandin F2α (free acid)) 100%(17-phenyl trinor Prostaglandin F2α ethyl amide) 80%(Latanoprost (free acid)) 1.50%(Latanoprost) 0.90%(5,6-trans Latanoprost) 0.40%(Fluprostenol) <0.01%(Fluprostenol isopropyl ester) <0.01%(2,3-dinor Fluprostenol) <0.01%(9-keto Fluprostenol) <0.01%(11-keto Fluprostenol) <0.01%(15-epi Latanoprost) <0.01%(tetranor-PGEM) <0.01%(tetranor-PGFM) <0.01%(Prostaglandin D2) <0.01%(Prostaglandin E2) <0.01%(Prostaglandin E2 Ethanolamide) <0.01%(6-keto Prostaglandin F1α) <0.01%(Prostaglandin F2α) <0.01%(Prostaglandin F2α Ethanolamide) <0.01%(5-trans Prostaglandin F2α) <0.01%(Thromboxane B2) <0.01%(Unoprostone) <0.01%(Unoprostone isopropyl ester) <0.01%
Origin
Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    17-phenyl trinor Prostaglandin F2α (17-phenyl trinor PGF2α) is a metabolically stable analog of PGF2α and is a potent agonist for the FP receptor. It binds to the FP receptor on ovine luteal cells with a relative potency of 756% compared to that of PGF2α.1 At the rat recombinant FP receptor expressed in CHO cells, 17-phenyl trinor PGF2α inhibits PGF2α binding with a Ki value of 1.1 nM.2 In human and animal models of glaucoma, FP receptor agonist activity corresponds very closely with intraocular hypotensive activity. 17-phenyl trinor PGF2α ethyl amide is an F-series prostaglandin analog which has been approved for use as an ocular hypotensive drug, sold under the Allergan trade name Bimatoprost.3 Whether 17-phenyl trinor PGF2α ethyl amide is a prodrug analogous to prostaglandin ester prodrugs such as latanoprost is currently controversial. There is some evidence that unmetabolized 17-phenyl trinor PGF2α is a weak FP receptor agonist.4 17-phenyl trinor PGF2α is converted by an amidase enzymatic activity in the bovine and human cornea to yield the corresponding free acid, with a conversion rate of about 40 µg/g corneal tissue/24 hours.5 Cayman’s 17-phenyl trinor PGF2α ELISA is a sensitive detection method for measuring both the free acid and ethyl amide forms of 17-phenyl trinor PGF2α. The assay is most appropriate for use when only one of the two forms is present. Samples containing mixtures of both the ethyl amide and free acid should be purified and the two compounds separated prior to ELISA analysis.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Balapure, A.K., Rexroad, C.E., Jr., Kawada, K., et al. Structural requirements for prostaglandin analog interaction with the ovine corpus luteum prostaglandin F2α receptor. Biochem. Pharmacol. 38(14), 2375-2381 (1989).

    2. Lake, S., Gullberg, H., Wahlqvist, J., et al. Cloning of the rat and human prostaglandin F2α receptors and the expression of the rat prostaglandin F2α receptor. FEBS Lett. 355(3), 317-325 (1994).

    3. Woodward, D.F., Krauss, A.H., Chen, J., et al. The pharmacology of bimatoprost (Lumigan™). Surv. Ophthalmol. 45(Suppl. 4), S337-S345 (2001).

    4. Sharif, N.A., Williams, G.W., and Kelly, C.R. Bimatoprost and its free acid are prostaglandin FP receptor agonists. Eur. J. Pharmacol. 432(2-3), 211-213 (2001).

    5. Maxey, K.M., Johnson, J., and LaBrecque, J. The hydrolysis of bimatoprost in corneal tissue generates a potent prostanoid FP receptor agonist. Surv. Ophthalmol. 47(Suppl. 1), S34-S40 (2002).