Complete drug discovery assay for screening COX-1 and COX-2 inhibitors
Features
  • Screen for inhibitors of ovine COX-1 or human COX-2
  • Directly measure PGF2α produced in the COX reaction
  • Assay 15 samples for COX-1 or COX-2 in duplicate
  • Plate-based colorimetric measurement (405-420 nm)
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COX (ovine/human) Inhibitor Screening Assay Kit

Item No. 560131

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Assay Range
3.9-500 pg/ml
Sensitivity
10 pg/ml
Origin
Animal/Sheep
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $534

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    Product Description

    The COX (ovine/human) Inhibitor Screening Assay directly measures PGF2α by SnCl2 reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via enzyme immunosorbent assay (ELISA) using an antiserum specific to PGF2α.

    This assay includes both ovine COX-1 and human recombinant COX-2 enzymes allowing the user to screen isozyme-specific inhibitors. This assay is an excellent tool which can be used for general inhibitor screening, or to eliminate false positive leads generated by less specific methods.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Singh, P., Kaur, J., Kaur, H., et al. Synergy of pysico-chemical and biological experiments for developing a cyclooxygenase-2 inhibitor. Sci. Rep. 8(1), 10005 (2018).

    Chao, J., Dai, Y., Cheng, H.-Y., et al. Improving the concentrations of the active components in the herbal tea ingredient, Uraria crinita: The effect of post-harvest oven-drying processing. Sci. Rep. 7:38763, (2017).

    Shaveta, Singh, A., Kaur, M., et al. Rational design, synthesis and evaluation of chromone-indole and chromone-pyrazole based conjugates: Identification of a lead for anti-inflammatory drug. Eur. J. Med. Chem. 77, 185-192 (2014).

    George, A., Chinnappan, S., Chintamaneni, M., et al. Anti-inflammatory effects of Polygonum minus (Huds) extract (Lineminus™) in in-vitro enzyme assays and carrageenan induced paw edema. BMC Complement. Altern. Med. 14:355, (2014).

    Macáková, K., Řeháková, Z., Mladěnka, P., et al. In vitro platelet antiaggregatory properties of 4-methylcoumarins. Biochimie 94(12), 2681-2686 (2012).

    Lee, J.O., Auger, C., Park, D.H., et al. An ethanolic extract of Lindera obtusiloba stems, YJP-14, improves endothelial dysfunction, metabolic parameters and physical performance in diabetic db/db mice. PLoS One 8(6), e65227 (2013).

    dos Santos, M.D., Chen, G., Almeida, M.C., et al. Effects of caffeoylquinic acid derivatives and C-flavonoid from Lychnophora ericoides on in vitro inflammatory mediator production. Nat. Prod. Commun. 5(5), 733-740 (2010).

    Wobst, I., Schiffmann, S., Birod, K., et al. Dimethylcelecoxib inhibits prostaglandin E2 production. Biochem. Pharmacol. 76(1), 62-69 (2008).