For direct quantification of cAMP from a variety of biological samples
Features
  • Measure acetylated or non-acetylated cAMP in cell lysates, tissue homogenates, plasma or urine
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure cAMP levels down to 0.1 pmol/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
  • Contains acetic anhydride
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Product Categories

Product Type

Assay & Reagent Kits
Assay Kits
ELISA

Research Area

Cell Biology
Cell Signaling
cAMP Signaling

Application

ELISA

Cyclic AMP ELISA Kit

Item No. 581001

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Adenosine 3',5'-cyclic mononucleotide
  • Adenosine 3',5'-cyclic monophosphate
  • cAMP EIA Kit
Assay Range
0.078-10 pmol/ml
Sensitivity
80% B/B0: 0.1 pmol/ml
Cross Reactivity
(Non-acetylated cAMP) 100%(Non-acetylated cGMP) 1.5%(Non-acetylated Adenosine) <0.01%(Non-acetylated AMP) <0.01%(Non-acetylated ATP) <0.01%(Acetylated cAMP) 100%(Acetylated cGMP) 0.69%(Acetylated adenosine) <0.01%(Acetylated AMP) <0.01%(2'3'-cAMP) <0.01%
Origin
Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Cyclic AMP is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase. Binding of certain ligands or hormones to their specific G-protein couple receptors activates GTP binding proteins (Gs or Gi) which either stimulate or inhibit adenylate cyclase. cAMP activates or inhibits various enzymes or cascade of enzymes by promoting their phosphorylation or dephosphorylation. The cAMP signal is neutralized by hydrolysis of cAMP to AMP by phosphodiesterases. Therefore, the concentration of cAMP in a cell is a function of the ratio of the rate of synthesis from ATP by adenylate cyclase and its rate of breakdown to AMP by specific phosphodiesterases.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Nam, G.S., Kim, S., Kwon, Y.-S., et al. A new function for MAP4K4 inhibitors during platelet aggregation and platelet-mediated clot retraction. Biochem. Pharmacol. 188, 114519 (2021).

    Holt, M.C., Ho, C.S., Morano, M.I., et al. Improved homology modeling of the human & rat EP4 prostanoid receptors. BMC Mol. Cell Biol. 20(1), 37 (2019).

    Flores-Costa, R., Alcaraz-Quiles, J., Titos, E., et al. The soluble guanylate cyclase stimulator IW-1973 prevents inflammation and fibrosis in experimental non-alcoholic steatohepatitis. Br. J. Pharmac. 175(6), 953-967 (2018).

    Maronde, E. Trehalose activates CRE-dependent transcriptional signaling in HT22 mouse hippocampal neuronal cells: A central role for PKA without cAMP elevation. Front. Mol. Neurosci. 11:386, (2018).

    Wei, W.-T., Chen, H., Wang, Z.-H., et al. Enhanced antitumor efficacy of gemcitabine by evodiamine on pancreatic cancer via regulating PI3K/Akt pathway. Int. J. Biol. Sci. 8(1), 1-14 (2012).

    Amisten, S., Meidute-Abaraviciene, S., Tan, C., et al. ADP mediates inhibition of insulin secretion by activation of P2Y13 receptors in mice. Diabetologia 53(9), 1927-1934 (2010).