Cyclic GMP ELISA Kit (without Acetic Anhydride)

Item № 581022
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
96 solid wells $270.00 0.00
96 strip wells $270.00 0.00
480 solid wells $1,013.00 0.00
480 strip wells $1,013.00 0.00

Pricing updated 2018-02-22. Prices are subject to change without notice.

Description
Features
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure acetylated or non-acetylated cGMP in cell lysates, tissue homogenates, plasma, or urine
  • Measure cGMP levels down to 0.1 pmol/ml
  • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
  • Kit does not contain acetic anhydride
Synonyms
  • cGMP EIA Kit (without Acetic Anhydride)
  • Guanosine 3',5'-cyclic mononucleotide
  • Guanosine 3',5'-cyclic monophosphate
  • Monosodium-GMP

cGMP is a key intracellular second messenger molecule which transduces cellular signaling events in response to a variety of hormones, autacoids and drugs. cGMP is synthesized from GTP by both membrane-bound and soluble guanlyate cyclase enzymes. The relative abundance of cGMP within a given cell can serve as a marker for activation by agonists acting through particulate guanylate cyclase at the cell surface, or intracellular activation of soluble guanylate cyclase by nitric oxide. Cayman’s cGMP Assay is a competitive ELISA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma or urine. The assay has a range from 0.23-30 pmol/ml and a sensitivity (80% B/B0) of approximately 1 pmol/ml. Since the antibody used in this assay was prepared against a cGMP-carrier protein conjugate, antibody binding is increased if an acetyl group is present on the 2’ hydroxyl group of the cGMP. The optional acetylation procedure for both samples and standards increases the sensitivity of the assay approximately ten-fold.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • cGMP EIA Kit (without Acetic Anhydride)
  • Guanosine 3',5'-cyclic mononucleotide
  • Guanosine 3',5'-cyclic monophosphate
  • Monosodium-GMP
Assay Range
0.23-30 pmol/ml
Sensitivity
80% B/B0: 1 pmol/ml
Mid-point
50% B/B0: 3-6 pmol/ml
Cross Reactivity
  • (Acetylated:)
  • (Non-Acetylated:)
  • (Acetylated cGMP) 100%
Show all 15

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Cyclic GMP ELISA Kit (without Acetic Anhydride)

View all analysis workbooks

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Contract Research Services

We work with scientists to support their research, drug discovery, and drug development needs through our expertise in the following research services:

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You may be eligible to receive a free sample of Cyclic GMP ELISA Kit (without Acetic Anhydride) under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640