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Estriol ELISA Kit

Item № 582281
product image
     96 solid wells $225.00 0.00
     96 strip wells $225.00 0.00
     480 solid wells $844.00 0.00
     480 strip wells $844.00 0.00

Pricing updated 2019-07-18. Prices are subject to change without notice.

  • Measure estriol, a major estrogen produced during pregnancy, in plasma and other sample matrices
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure estriol levels down to 4 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
  • Estriol EIA Kit
  • E3
  • 16α-hydroxy-17β-Estradiol
  • 1,3,5(10)-Estratriene-3,16α,17β-triol

Estriol, a metabolite of 17β-estradiol and a major estrogen produced during pregnancy, has historically been used as an indicator of fetal well-being. In the final weeks before parturition, estriol levels increase significantly. In a longitudinal study in healthy pregnant women, total plasma estriol levels increased from <10 ng/ml at 8-10 weeks gestation to approximately 150 ng/ml at week 38.1 The majority of the estriol synthesized in the later stages of pregnancy originates from fetal dehydroepiandrosterone sulfate (DHEAS) and serves as a direct marker of fetal adrenal gland activity.2 In the 1960s and 1970s plasma and urine were used to measure estriol. However, saliva contains primarily unbound and unconjugated estriol, the biologically active form of the hormone, and therefore is currently a commonly used biological fluid for monitoring estriol levels.2,3 Plasma levels of estriol in males and non-pregnant females is less than 2 ng/ml.4

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Estriol EIA Kit
  • E3
  • 16α-hydroxy-17β-Estradiol
  • 1,3,5(10)-Estratriene-3,16α,17β-triol
Assay Range
0.9-2,000 pg/ml
80% B/B0: 4 pg/ml (4°C for 18 hours); 12 pg/ml (RT for 2 hours)
50% B/B0: 25-60 pg/ml (4°C for 18 hours)
Cross Reactivity
  • (Estriol) 100%
  • (Estriol-3-glucuronide) 6%
  • (Estriol-16-glucuronide) 1.2%
Show all 11
  • Animal/Bovine
  • Animal/Eel
  • Animal/Mouse
  • Animal/Rabbit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Peter, M., Dörr, H.G., and Sippell, W.G. Changes in the concentrations of dehydroepiandrosterone sulfate and estriol in maternal plasma during pregnancy: A longitudinal study in healthy women throughout gestation and at term Hormone Research 42, 278-281 (1994).

2. Goodwin, T.M. A role for estriol in human labor, term and preterm American Journal of Obstetics and Gynecology 180, S208-S213 (1999).

3. Voss, H.F. Hormonal pathways of preterm birth American Journal of Obstetics and Gynecology 180, S226-S231 (1999).

4. Steroid levels in humans (1999).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Estriol ELISA Kit

View all analysis workbooks

View the pre-configured analysis tool for Estriol ELISA Kit on MyAssays.com

Contact Us
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  • Direct Phone: (734) 975-3888
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Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

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