For the quantification of progesterone in plasma and other sample matrices
Features
  • Measure progesterone in plasma and other sample matrices
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure progesterone levels down to 10 pg/ml
  • Rapid assay; get results in under 4 hours
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Progesterone ELISA Kit

Item No. 582601

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Progesterone EIA Kit
Assay Range
7.8-1,000 pg/ml
Sensitivity
80% B/B0: 10 pg/ml
Cross Reactivity
(Progesterone) 100%(Pregnenolone) 14.0%(17β-Estradiol) 7.2%(5β-Pregnan-3α-ol-20-one) 6.7%(17α-Hydroxyprogesterone) 3.6%(17α-Hydroxypregnenolone) 0.44%(Corticosterone) 0.26%(Testosterone) <0.05%(17α-Estradiol) <0.01%(Estriol) <0.01%(Estrone) <0.01%(5α-Pregnan-3α,20α-diol) <0.01%(5β-Pregnan-3α,20α-diol) <0.01%(5β-Pregnan-3α,20α-diol glucuronide) <0.01%(Promegestone) <0.01%(DHEA) 0.015%(Cortisol) 0.006%(DHEA Sulfate) 0.006%
Origin
Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Progesterone, along with pregnenolone, is the biosynthetic precursor of all other steroid hormones. Progesterone is synthesized from cholesterol by the sequential action of desmolase in the mitochondria, which produces pregnenolone, followed by Δ4,5-isomerase in the outer mitochondrial membrane and smooth endoplasmic reticulum of steroid-secreting cells.1 The main function of progesterone is to prepare the uterine lining for implantation of a fertilized ovum and to maintain pregnancy. Measurement of serum or plasma progesterone levels are used as an index to monitor ovulation and investigate luteal function. Plasma concentrations of progesterone are approximately 0.2-0.8 ng/ml and 4-20 ng/ml during the follicular phase and luteal phase of the menstrual cycle, respectively.1 Salivary concentrations of progesterone are approximately 100-fold lower than those found in plasma.2

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. Endocrinology and Metabolism 973-1015 (1995).

    2. Tallon, D.F., Gosling, J.P., Buckley, P.M., et al. Direct solid-phase enzyme immunoassay of progesterone in saliva. Clin. Chem. 30, 1507-1511 (1984).

    Product Citations

    Moutard, L., Goudin, C., Jaeger, C., et al. Steroidogenesis and androgen/estrogen signaling pathways are altered in in vitro matured testicular tissues of prepubertal mice. Elife 12, RP85562 (2023).

    Taves, M.D., Donahue, K.M., Bian, J., et al. Aire drives steroid hormone biosynthesis by medullary thymic epithelial cells. Sci. Immunol. 8(86), eabo7975 (2023).

    Ashry, M., Folger, J.K., Rajput, S.K., et al. FSH stimulated bovine granulosa cell steroidogenesis involves both canonical and noncanonical WNT signaling. Domest. Anim. Endocrinol. 78, 106678 (2022).

    Shin, Y.Y., Jeong, J.S., Park, M.-N., et al. Regulation of steroid hormones in the placenta and serum of women with preeclampsia. Mol. Med. Rep. 17(2), 2681-2688 (2018).

    Farhat, A., Philibert, P., Sultan, C., et al. Hematopoietic-Prostaglandin D2 synthase through PGD2 production is involved in the adult ovarian physiology. J. Ovarian Res. 4, 3 (2011).