For the quantification of 11-keto testosterone in plasma and other sample matrices
Features
  • Detect the primary androgenic steroid in fish
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 11-keto testosterone levels down to 1.3 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
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11-keto Testosterone ELISA Kit

Item No. 582751

Exempt Product (Canada)
Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 11-KT EIA Kit
Assay Range
0.78-100 pg/ml
Sensitivity
80% B/B0: 1.3 pg/ml
Cross Reactivity
(11-keto Testosterone) 100%(Adrenosterone) 2.9%(4-Androsten-11β,17β-diol-3-one) 0.01%(5α-Androstan-17β-ol-3-one) <0.01%(5α-Androsten-3β,17β-diol) <0.01%(Testosterone) <0.01%
Origin
Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit
Regulatory Information
Canada Exempt Notification
This product is registered as a Canada Test Kit - no additional Health Canada import paperwork or permits required.
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Bioanalysis & Assay Development Services
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    Product Description

    While testosterone is the primary androgenic male steroid found in mammals, 11-keto testosterone (11-KT) is a second key androgenic steroid found in fish. It occurs in males together with testosterone in amounts which vary from less than 1 ng/ml to as much as 50-100 ng/ml, depending on the species and the stage of the reproductive cycle. In the sea bass, testosterone concentrations are generally higher than 11-KT, with peak levels found after the spawning season. 11-KT, on the other hand, remains at levels less than 1 ng/ml but rises abruptly to 4-6 ng/ml during spermiation at the height of the spawning season.1 11-KT also shows individual variations in Arctic char, with dominant males having significantly higher 11-KT levels.2

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Prat, F., Zanuy, S., Carrillo, M., et al. Seasonal changes in plasma levels of gonadal steroids of sea bass, Dicentrarchus labrax L. Gen. Comp. Endocrinol. 78, 361-373 (1990).

    2. Elofsson, U.O.E., Mayer, I., Damsgård, B., et al. Intermale competition in sexually mature arctic charr: effects on brain monoamines, endocrine stress responses, sex hormone levels, and behavior. Gen. Comp. Endocrinol. 118, 450-460 (2000).

    Product Citations

    Liu, X., Lu, X., Hong, J., et al. Effects of long-term exposure to TDCPP in zebrafish (Danio rerio) - alternations of hormone balance and gene transcriptions along hypothalamus-pituitary axes. Animal Model Exp. Med. 5(3), 239-247 (2022).

    Zhu, Y., Hua, R., Zhou, Y., et al. Chronic exposure to mono-(2-ethylhexyl)-phthalate causes endocrine disruption and reproductive dysfunction in zebrafish. Environ. Toxicol. Chem. 35(8), 2117-2124 (2016).

    Liu, X., Ji, K., and Choi, K. Endocrine disruption potentials of organophosphate flame retardants and related mechanisms in H295R and MVLN cell lines and in zebrafish. Aquat. Toxicol. 114-115, 173-181 (2012).