Interleukin-1β (human) ELISA Kit

Interleukin 1 (IL-1) is a name for a family of proteins which includes IL-1α, IL-1β, ILra (Interleukin receptor antagonist) and IL-18. IL-1β is synthesized as 269 amino acid pro-cytokine that is enzymatically cleaved by IL-1β-converting enzyme (ICE) into the active 154 amino acid, 17 kDa peptide mature peptide.^1,2 IL-1β is produced by many cell types including macrophages, monocytes, astrocytes, endothelial cells, smooth muscle cells, keratinocytes, adipocytes, T cells, and eosinophils.³ Although IL-1α and IL-1β exhibit only 26% amino acid identity,¹ they bind to the same cell-surface receptors, IL-1 RI and IL-1 RII, present on a variety of cell types involved in immune or inflammatory responses.^4,5,6 Normal production of IL-1 is critical for mediating host responses to infection and injury.^2,3 Disease states in which IL-1 actively participates include inflammatory diseases, such as arthritis, inflammatory bowel disease, and shock, as well as arteriosclerosis, allergic disease, and some types of cancer.^3,7

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

1. March, C.J., Mosley, B., Larsen, A., et al. Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature 315, 641-647 (1985).

2. Thornberry, N.A., Bull, H.G., Calaycay, J.R., et al. A novel heterodimeric cysteine protease is required for interleukin-1β processing in monocytes. Nature 356(6372), 768-774 (1992).

3. Dinarello, C.A. Interleukin-1 and the pathogenesis of the acute-phase response. N. Engl. J. Med. 311, 1413-1418 (1984).

4. Sims, J.E., March, C.J., Cosman, D., et al. cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily. Science 241, 585-589 (1988).

5. Sims, J.E., Acres, R.B., Grubin, C.E., et al. Cloning the interleukin 1 receptor from human T cells. Proc. Natl. Acad. Sci. USA 86, 8946-8950 (1989).

6. McMahan, C.J., Slack, J.L., Mosley, B., et al. A novel IL-1 receptor, cloned from B cells by mammaliam expression, is expressed in many cell types. EMBO J. 10(10), 2821-2832 (1991).

7. Rosenwasser, L.J. Biologic activities of IL-1 and its role in human disease. J. Allergy Clin. Immunol. 102, 344-350 (1998).

Xiao, W., Yu, A., Liu, D., et al. Ligustilide treatment promotes functional recovery in a rat model of spinal cord injury via preventing ROS production. Int. J. Clin. Exp. Pathol. 8(10), 12005-12013 (2015).