Angiotensin II EIA Kit | Cayman Chemical
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Angiotensin II EIA Kit

Item № 589301
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 wells $530.00 0.00

Pricing updated 2018-08-14. Prices are subject to change without notice.

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Description
Features
  • Measure angiontensin II in mammalian plasma, serum, and cell culture supernatant samples
  • Assay 33 samples in duplicate
  • Incubations: 1 hour | Overnight
  • Angiotensin II Inhibitor Cocktail (Item No. 9000681) and phenyl cartridges (Item No. 400048) available for sample storage and clean up
Synonyms
  • Angiotensin II ELISA Kit

Angiotensin II is a primary reactive vasoconstrictor, the main stimulus for aldosterone release, and one of the causative factors of chronic hypertension. The active angiotensin II octapeptide is released via a tightly controlled series of prohormones and proteases as diagrammed below. Normal human plasma angiotensin II levels are from 10-30 pg/ml when measured at rest in the supine position; they increase on standing, exercise, dehydration, or sodium depletion. The unique, patented 'Immobilized Antigen' technology of this angiotensin II immunometric assay allows reliable detection of 1-2 pg/ml, or as little as 10% of the normal human plasma concentration. [Bertin Catalog No. A05880]

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • Angiotensin II ELISA Kit
Specificity
  • (100%) Angiotensin II
  • (36%) Angiotensin III
  • (33%) Angiotensin 3-8
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Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 6 months
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Citations

Tufiño, C., Villanueva-López, C., Ibarra-Barajas, M., et al. Experimental gestational diabetes mellitus induces blunted vasoconstriction and functional changes in the rat aorta. Biomed. Res. Int. 329634 (2014).

Carroll, W.X., Kalupahana, N.S., Booker, S.L., et al. Angiotensinogen gene silencing reduces markers of lipid accumulation and inflammation in cultured adipocytes. Front. Endocrinol. (Lausanne) 4, 10 (2013).

Marampon, F., Gravina, G.L., Scarsella, L., et al. Angiotensin-converting-enzyme inhibition counteracts angiotensin II-mediated endothelial cell dysfunction by modulating the p38/SirT1 axis. J. Hypertens. 31(10), 1972-1983 (2013).

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Technical Support
Analysis Tools

View our analysis workbook for Angiotensin II EIA Kit

View all analysis workbooks

FAQ

Is the Angiotensin II EIA Kit suitable for use with rat plasma and supernatant of cell culture?

The sequence for angiotensin II is conserved across mammalian species. Therefore, this kit will work with rat samples, however purification is required.

Is purification needed for plasma samples prior to use in the Angiotensin II EIA Kit?

Yes, plasma samples need purification prior to use in the Angiotensin II EIA Kit. The chemicals are available from Sigma: o-Phenanthroline Catalog number 33510, p-hydroxymercuribenzoic acid Catalog number 55540 Pepstatin A Catalog number 77170. The phenyl cartridge is from Phenomenex/Strata. The catalog numbers are: 1 ml Cat 8B S006 EAK , 3 ml Cat 8B S006 HBJ.

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You may be eligible to receive a free sample of Angiotensin II EIA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

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Fax: (734) 971-3640