DNA/RNA Oxidative Damage ELISA Kit | Cayman Chemical
We collect cookies for vital website function and to better serve our customers. By continuing to browse you agree to the storing of cookies on your device. See our privacy policy for details.

DNA/RNA Oxidative Damage ELISA Kit

Item № 589320
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 solid wells $495.00 0.00
     96 strip wells $495.00 0.00
     480 solid wells $1,856.00 0.00
     480 strip wells $1,856.00 0.00

Pricing updated 2018-05-27. Prices are subject to change without notice.

Description
Features
  • Measure major oxidative damage markers, 8-hydroxy-2’-deoxyguanosine​​, 8-hydroxyguanosine, and 8-hydroxyguanine in urine, cell culture medium, cell lysates, tissue samples, saliva, and plasma/serum samples
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Assay Range: 10.3-3,000 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • DNA/RNA Oxidative Damage EIA Kit
  • 8-hydroxy-2'-Deoxyguanosine
  • 8-OH-DG
  • 8-OH-2dG

Both DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer. The repair processes that are initiated to correct this damage release multiple oxidized guanine species into the urine. Cayman Chemical has developed this immunoassay for the measurement of DNA/RNA oxidative damage that detects all three oxidized guanine species; 8-hydroxy-2'-deoxyguanosine from DNA, 8-hydroxyguanosine from RNA, and 8-hydroxyguanine from either DNA or RNA. Some commercial vendors offer immunoassays that detect only 8-hydroxy-2'-deoxyguanosine, and not the other two molecules. The advantage of the Cayman kit is that it captures a more complete set of biologically relevant products of oxidative damage than do assays that are restricted to analysis of only 8-hydroxy-2'-deoxyguanosine. However, because Cayman’s kit recognizes more than 8-hydroxy-2'-deoxyguanosine, it is not valid to compare the results from the Cayman ELISA Kit to an LC/MS analysis of 8-hydroxy-2'-deoxyguanosine. The ELISA value will always be significantly higher than LC/MS because the ELISA also detects 8-hydroxyguanosine and 8-hydroxyguanine.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Recommended Products

Related Products
View Related Product Categories
Technical Information
Synonyms
  • DNA/RNA Oxidative Damage EIA Kit
  • 8-hydroxy-2'-Deoxyguanosine
  • 8-OH-DG
  • 8-OH-2dG
Assay Range
10.3-3,000 pg/ml
Sensitivity
80% B/B0: 30 pg/ml
Mid-point
50% B/B0: 90-140 pg/ml
Cross Reactivity
  • (8-hydroxy-2’-deoxyguanosine) 100%
  • (8-hydroxyguanosine) 38%
  • (8-hydroxyguanine) 23%
Show all 4

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for DNA/RNA Oxidative Damage ELISA Kit

View all analysis workbooks

View the pre-configured analysis tool for DNA/RNA Oxidative Damage ELISA Kit on MyAssays.com

FAQ

The the level of 8-OH-dG has been estimated in cultured cells after extracting total DNA. What is the best way to normalize the data for accurate comparison between treatments?

It is more meaningful to normalize measured values to the total number of cells, or the amount of purified DNA rather than as a concentration of 8-OH-dG only.

Has the 8-hydroxy-2-deoxy Guanosine EIA Kit been validated for muscle tissue or whole blood (leukocyte DNA) samples?

The 8-hydroxy-2-deoxy Guanosine EIA Kit has not been validated for those exact sample types, but has been validated for cell lysates and tissue homogenates in general. Theoretically, the kit should work just fine with any purified DNA sample.

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Contract Research Services

We work with scientists to support their research, drug discovery, and drug development needs through our expertise in the following research services:

Promotions

View all of our current Promotions

You may be eligible to receive a free sample of DNA/RNA Oxidative Damage ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640