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|96 solid wells||$575.00||0.00|
|96 strip wells||$575.00||0.00|
|480 solid wells||$2,156.00||0.00|
|480 strip wells||$2,156.00||0.00|
Pricing updated 2019-03-20. Prices are subject to change without notice.
DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer.1,2,3 Guanine is the base is most prone to oxidation, and the repair processes initiated to correct the damage release multiple oxidized guanine species into the urine, including 8-hydroxyguanosine (8-OHG), 8-hydroxy-2’-deoxyguanosine (8-OHdG), and 8-hydroxyguanine. Some commercial vendors offer immunoassays that detect only 8-OHdG. Cayman's DNA/RNA Oxidative Damage (High Sensitivity) ELISA Kit is a competitive assay that can be used to measure 8-OHdG, 8-OHG, and 8-hydroxyguanine. Since the antibody used in the assay recognizes damaged nucleic acid species other than 8-OHdG, the value obtained with our assay will be higher than that obtained by competitor ELISAs or by LC/MS analysis that measure a single species. As such, caution is recommended when comparing results obtained from this kit to those obtained by other methods.
Warning - this product is not for human or veterinary use.
View the Cayman Structure Database for chemical structure definitions for many Cayman products
Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.
Gan, W., Nie, B., Shi, F., et al. Age-
Shi, F., Nie, B., Gan, W., et al. Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-
Roszkowski, K., and Olinski, R. Urinary 8-
Jamali, F., Kavoosi, G., Safavi, M., et al. In-
Withee, E.D., Tippens, K.M., Dehen, R., et al. Effects of methylsulfonylmethane (MSM) on exercise-
Salehi, F., Behboudi, H., Kavoosi, G., et al. Monitoring ZEO apoptotic potential in 2D and 3D cell cultures and associated spectroscopic evidence on mode of interaction with DNA Sci. Rep. 7(1), 2553 (2017).Show all 10
All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.
View all analysis workbooks
View the pre-configured analysis tool for DNA/RNA Oxidative Damage (High Sensitivity) ELISA Kit on MyAssays.com
The the level of 8-OH-dG has been estimated in cultured cells after extracting total DNA. What is the best way to normalize the data for accurate comparison between treatments?
It is more meaningful to normalize measured values to the total number of cells, or the amount of purified DNA rather than as a concentration of 8-OH-dG only.
Has the 8-hydroxy-2-deoxy Guanosine EIA Kit been validated for muscle tissue or whole blood (leukocyte DNA) samples?
The 8-hydroxy-2-deoxy Guanosine EIA Kit has not been validated for those exact sample types, but has been validated for cell lysates and tissue homogenates in general. Theoretically, the kit should work just fine with any purified DNA sample.