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DNA/RNA Oxidative Damage (High Sensitivity) ELISA Kit

Item № 589320
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 solid wells $575.00 0.00
     96 strip wells $575.00 0.00
     480 solid wells $2,156.00 0.00
     480 strip wells $2,156.00 0.00

Pricing updated 2019-05-26. Prices are subject to change without notice.

Description
Features
  • Measure major oxidative damage markers, 8-hydroxy-2’-deoxyguanosine​​, 8-hydroxyguanosine, and 8-hydroxyguanine in urine, cell culture medium, cell lysates, tissue samples, saliva, and plasma/serum samples
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Assay Range: 10.3-3,000 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • DNA/RNA Oxidative Damage (High Sensitivity) EIA Kit
  • 8-hydroxy-2'-Deoxyguanosine
  • 8-OH-DG
  • 8-OH-2dG

DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer.1,2,3 Guanine is the base is most prone to oxidation, and the repair processes initiated to correct the damage release multiple oxidized guanine species into the urine, including 8-hydroxyguanosine (8-OHG), 8-hydroxy-2’-deoxyguanosine (8-OHdG), and 8-hydroxyguanine. Some commercial vendors offer immunoassays that detect only 8-OHdG. Cayman's DNA/RNA Oxidative Damage (High Sensitivity) ELISA Kit is a competitive assay that can be used to measure 8-OHdG, 8-OHG, and 8-hydroxyguanine. Since the antibody used in the assay recognizes damaged nucleic acid species other than 8-OHdG, the value obtained with our assay will be higher than that obtained by competitor ELISAs or by LC/MS analysis that measure a single species. As such, caution is recommended when comparing results obtained from this kit to those obtained by other methods.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • DNA/RNA Oxidative Damage (High Sensitivity) EIA Kit
  • 8-hydroxy-2'-Deoxyguanosine
  • 8-OH-DG
  • 8-OH-2dG
Assay Range
10.3-3,000 pg/ml
Sensitivity
80% B/B0: 30 pg/ml
Mid-point
50% B/B0: 90-140 pg/ml
Cross Reactivity
  • (8-hydroxy-2’-deoxyguanosine) 100%
  • (8-hydroxyguanosine) 38%
  • (8-hydroxyguanine) 23%
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Origin
  • Animal/Bovine
  • Animal/Eel
  • Animal/Goat
  • Animal/Mouse

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Gan, W., Nie, B., Shi, F., et al. Age-dependent increases in the oxidative damage of DNA, RNA, and their metabolites in normal and senescence-accelerated mice analyzed by LC-MS/MS: Urinary 8-oxoguanosine as a novel biomarker of aging Free Radical Biology & Medicine 52, 1700-1707 (2012).

2. Shi, F., Nie, B., Gan, W., et al. Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-oxoguanosine in urine is a useful marker for aging Free Radical Research 46(9), 1093-1098 (2012).

3. Roszkowski, K., and Olinski, R. Urinary 8-oxoguanine as a predictor of survival in patients undergoing radiotherapy Cancer Epidemiology, Biomarkers & Prevention 21, 629-634 (2012).

Product Citations

Jamali, F., Kavoosi, G., Safavi, M., et al. In-vitro evaluation of apoptotic effect of OEO and thymol in 2D and 3D cell cultures and the study of their interaction mode with DNA Sci. Rep. 8(1), 15787 (2018).

Withee, E.D., Tippens, K.M., Dehen, R., et al. Effects of methylsulfonylmethane (MSM) on exercise-induced oxidative stress, muscle damage, and pain following a half-marathon: a double-blind, randomized, placebo-controlled trial J. Int. Soc. Sports Nutr. 14:24, (2017).

Salehi, F., Behboudi, H., Kavoosi, G., et al. Monitoring ZEO apoptotic potential in 2D and 3D cell cultures and associated spectroscopic evidence on mode of interaction with DNA Sci. Rep. 7(1), 2553 (2017).

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Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for DNA/RNA Oxidative Damage (High Sensitivity) ELISA Kit

View all analysis workbooks

View the pre-configured analysis tool for DNA/RNA Oxidative Damage (High Sensitivity) ELISA Kit on MyAssays.com

FAQ

The the level of 8-OH-dG has been estimated in cultured cells after extracting total DNA. What is the best way to normalize the data for accurate comparison between treatments?

It is more meaningful to normalize measured values to the total number of cells, or the amount of purified DNA rather than as a concentration of 8-OH-dG only.

Has the 8-hydroxy-2-deoxy Guanosine EIA Kit been validated for muscle tissue or whole blood (leukocyte DNA) samples?

The 8-hydroxy-2-deoxy Guanosine EIA Kit has not been validated for those exact sample types, but has been validated for cell lysates and tissue homogenates in general. Theoretically, the kit should work just fine with any purified DNA sample.

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  • Direct Phone: (734) 975-3888
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