DNA/RNA Oxidative Damage (High Sensitivity) ELISA Kit

DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer.^1,2,3 Guanine is the base is most prone to oxidation, and the repair processes initiated to correct the damage release multiple oxidized guanine species into the urine, including 8-hydroxyguanosine (8-OHG), 8-hydroxy-2’-deoxyguanosine (8-OHdG), and 8-hydroxyguanine. Some commercial vendors offer immunoassays that detect only 8-OHdG. Cayman's DNA/RNA Oxidative Damage (High Sensitivity) ELISA Kit is a competitive assay that can be used to measure 8-OHdG, 8-OHG, and 8-hydroxyguanine. Since the antibody used in the assay recognizes damaged nucleic acid species other than 8-OHdG, the value obtained with our assay will be higher than that obtained by competitor ELISAs or by LC/MS analysis that measure a single species. As such, caution is recommended when comparing results obtained from this kit to those obtained by other methods.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

1. Gan, W., Nie, B., Shi, F., et al. Age-dependent increases in the oxidative damage of DNA, RNA, and their metabolites in normal and senescence-accelerated mice analyzed by LC-MS/MS: Urinary 8-oxoguanosine as a novel biomarker of aging. Free Radic. Biol. Med. 52, 1700-1707 (2012).

2. Shi, F., Nie, B., Gan, W., et al. Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-oxoguanosine in urine is a useful marker for aging. Free Radic. Res. 46(9), 1093-1098 (2012).

3. Roszkowski, K., and Olinski, R. Urinary 8-oxoguanine as a predictor of survival in patients undergoing radiotherapy. Cancer Epidemiol. Biomarkers Prev. 21, 629-634 (2012).

Jamali, F., Kavoosi, G., Safavi, M., et al. In-vitro evaluation of apoptotic effect of OEO and thymol in 2D and 3D cell cultures and the study of their interaction mode with DNA. Sci. Rep. 8(1), 15787 (2018).

Vieira, G.d.L.T., Lossie, A.C., Lay, D.C., Jr., et al. Preventing, treating, and predicting barbering: A fundamental role for biomarkers of oxidative stress in a mouse model of Trichotillomania. PLoS One 12(4), e0175222 (2017).

Withee, E.D., Tippens, K.M., Dehen, R., et al. Effects of methylsulfonylmethane (MSM) on exercise-induced oxidative stress, muscle damage, and pain following a half-marathon: a double-blind, randomized, placebo-controlled trial. J. Int. Soc. Sports Nutr. 14:24, (2017).

Salehi, F., Behboudi, H., Kavoosi, G., et al. Monitoring ZEO apoptotic potential in 2D and 3D cell cultures and associated spectroscopic evidence on mode of interaction with DNA. Sci. Rep. 7(1), 2553 (2017).

Vieira, G.L.T., Lossie, A.C., Lay, D.C., Jr., et al. Preventing, treating, and predicting barbering: A fundamental role for biomarkers of oxidative stress in a mouse model of Trichotillomania. PLoS One 12(4), e0175222 (2017).

Zhou, H.-x., Ban, H., Hou, L.-M., et al. Protective effects of hydrogen gas on experimental acute pancreatitis. PLoS One 11(4), e0154483 (2016).

Vriens, K., Christen, S., Parik, S., et al. Evidence for an alternative fatty acid desaturation pathway increasing cancer plasticity. Nature 566(7744), 403-406 (2019).

Crinigan, C., Calhoun, M., and Sewazea, K.L. Short-term high fat intake does not significantly alter markers of renal function or inflammation in young male sprague-dawley rats. J. Nutr. Metab. 157520, (2015).

Dhall, S., Do, D., Garcia, M., et al. A novel model of chronic wounds: Importance of redox imbalance and biofilm-forming bacteria for establishment of chronicity. PLoS One 9(10), e109848 (2014).

Schisler, J.C., Grevengoed, T.J., Pascual, F., et al. Cardiac energy dependence on glucose increases metabolites related to glutathione and activates metabolic genes controlled by mechanistic target of rapamycin. J. Am. Heart Assoc. 4(2), e001136 (2015).

Wong, W.-L., Abdulla, M.A., Chua, K.-H., et al. Hepatoprotective effects of Panus giganteus (Berk.) corner against thioacetamide- (TAA-) induced liver injury in rats. Evid. Based Complement. Alternat. Med. 170303, (2012).