A competitive ELISA for the quantification of 5-methyl-2'-deoxy cytidine
Features
  • Measure 5-methyl-2’-deoxy cytidine in urine, plasma, cell lysates, and tissue culture supernatants
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 5-methyl-2’-deoxy cytidine levels down to 3 ng/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
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DNA Methylation ELISA Kit

Item No. 589324

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 5-Methyl-2'-deoxycytidine EIA Kit
Assay Range
1.17-150 ng/ml
Sensitivity
80% B/B0: ~3 ng/ml
Cross Reactivity
(5-Methyl-2'-deoxycytidine) 100%(5-Methylcytidine) 20%(2-Deoxycytidine) 0.1%(Cytidine) 0.1%(Thymidine) <0.01%
Origin
Animal/Bovine, Animal/Eel, Animal/Goat, Animal/Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    96 solid wells

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    Cayman Chemical
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    Product Description

    DNA methylation is an important epigenetic process regulating gene expression. Methylation occurs on carbon 5 of 2'-deoxy-cytidine yielding the modified base 5-methyl-2'-deoxy cytidine. The methylation pattern of cells is tightly regulated during development with the methylation profile being transmitted from parent to daughter cells during cell division. Methylation results in long-term silencing of genes, while unmethylated regions of DNA can be actively transcribed. Global changes in methylation can be quantified by measuring plasma or urinary levels of 5-methyl-2'-deoxy cytidine. These changes in methylation can provide valuable information about cancer status of an individual. For example, patients with leukemia excrete significantly elevated levels of 5-methyl-2'-deoxy cytidine compared to healthy individuals. Global methylation within tissues can be measured in a similar manner, allowing study of tissue-specific changes that occur as a result of differentiation, aging, or carcinogenesis. Cayman’s DNA Methylation ELISA Kit is a competitive assay that can be used for the quantification of 5-methyl-2'-deoxy cytidine in urine, culture supernatants, plasma, and other sample matrices. The ELISA typically displays IC50 (50% B/B0) and IC80 (80% B/B0) values of approximately 12 and 3 ng/ml, respectively.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.