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Prostaglandin D Synthase (hematopoietic-type) FP-Based Inhibitor Screening Assay Kit - Green

Item № 600007
product image
     384 wells $356.00 0.00
     1920 wells $1,335.00 0.00

Pricing updated 2019-06-16. Prices are subject to change without notice.

  • Screen for inhibitors of H-PGDS
  • Designed for high-throughput screening
  • Includes HQL-79 as a positive control
  • Plate-based fluorescence polarization measurement (ex 470 nm, em 530 nm)
  • Prostaglandin D Synthase (hematopoietic-type) Fluorescence Polarization-Based Inhibitor Screening Assay Kit - Green
  • H-PGDS FP-Based Inhibitor Screening Assay Kit - Green

Prostaglandin D2 (PGD2) is synthesized by hematopoietic-type PGD-synthase (H-PGDS) in mast cells and is released in large quantities during allergic and asthmatic anaphylaxis. H-PGDS is therefore a key target for development of selective, potent inhibitors for therapeutic use against these diseases. Cayman’s H-PGDS FP-Based Inhibitor Screening Assay Kit - Green provides a rapid, accurate assay for screening H-PGDS inhibitors. In this assay, a H-PGDS inhibitor-fluorescein conjugate serves as a specific fluorescent probe for the enzyme. Displacement of the probe by any unlabeled H-PGDS inhibitor leads to a decrease in the fluorescence polarization (FP) state of the probe, providing a direct signal for binding of the inhibitor to the active site of the enzyme. This assay is robust (Z’ of 0.75) and eliminates the need for the traditional multistep assay that requires the use of highly unstable PGH2. The assay has been validated using several known inhibitors of H-PGDS with IC50 values ranging from nanomolar to millimolar concentrations.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Prostaglandin D Synthase (hematopoietic-type) Fluorescence Polarization-Based Inhibitor Screening Assay Kit - Green
  • H-PGDS FP-Based Inhibitor Screening Assay Kit - Green

Warning - this product is not for human or veterinary use.

Shipping & Storage
Dry ice in continental US; may vary elsewhere
≥ 1 year
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