Prostaglandin D Synthase (hematopoietic-type) FP-Based Inhibitor Screening Assay Kit - Green

Item № 600007
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
384 wells $356.00 0.00
1920 wells $1,335.00 0.00

Pricing updated 2018-02-21. Prices are subject to change without notice.

Description
Features
  • Screen for inhibitors of H-PGDS
  • Designed for high-throughput screening
  • Includes HQL-79 as a positive control
  • Plate-based fluorescence polarization measurement (ex 470 nm, em 530 nm)
Synonyms
  • Prostaglandin D Synthase (hematopoietic-type) Fluorescence Polarization-Based Inhibitor Screening Assay Kit - Green
  • H-PGDS FP-Based Inhibitor Screening Assay Kit - Green

Prostaglandin D2 (PGD2) is synthesized by hematopoietic-type PGD-synthase (H-PGDS) in mast cells and is released in large quantities during allergic and asthmatic anaphylaxis. H-PGDS is therefore a key target for development of selective, potent inhibitors for therapeutic use against these diseases. Cayman’s H-PGDS FP-Based Inhibitor Screening Assay Kit - Green provides a rapid, accurate assay for screening H-PGDS inhibitors. In this assay, a H-PGDS inhibitor-fluorescein conjugate serves as a specific fluorescent probe for the enzyme. Displacement of the probe by any unlabeled H-PGDS inhibitor leads to a decrease in the fluorescence polarization (FP) state of the probe, providing a direct signal for binding of the inhibitor to the active site of the enzyme. This assay is robust (Z’ of 0.75) and eliminates the need for the traditional multistep assay that requires the use of highly unstable PGH2. The assay has been validated using several known inhibitors of H-PGDS with IC50 values ranging from nanomolar to millimolar concentrations.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • Prostaglandin D Synthase (hematopoietic-type) Fluorescence Polarization-Based Inhibitor Screening Assay Kit - Green
  • H-PGDS FP-Based Inhibitor Screening Assay Kit - Green

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

Technical Support
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  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of Prostaglandin D Synthase (hematopoietic-type) FP-Based Inhibitor Screening Assay Kit - Green under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640