Pricing updated 2018-08-14. Prices are subject to change without notice.
Reactive oxygen species, such as hydrogen peroxide (H2O2) and superoxide are generated by phagocytes and participate in damaging invading miccroorganisms or other biologic targets.1 Cayman's H2O2 Cell-Based Assay Kit provides a simple fluorometric method for the sensitive quantitation of extracellular H2O2 produced by cultured cells. H2O2 is detected using ADHP (10-acetyl-3,7-dihydroxyphenoxazine), a highly sensitive and stable probe for H2O2.2 In the presence of horseradish peroxidase, ADHP reacts with H2O2 with a 1:1 stoichiometry to produce highly fluorescent resorufin.3 Resorufin fluorescence can be read using an excitation wavelength between 530-560 nm and an emission wavelength of 590 nm. Catalase, an H2O2 scavenger, is included in the kit for checking specificity of the assay.
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1. Rosen, G.M., Pou, S., Ramos, C.L., et al. Free radicals and phagocytic cells. The FASEB Journal 9, 200-209 (1995).
Zhou, M., Diwu, Z., Panchuk-
3. Amundson, D.M., and Zhou, M. Fluorometric method for the enzymatic determination of cholesterol. Journal of Biochemical and Biophysical Methods 38, 43-52 (1999).