Hydrogen Peroxide Cell-Based Assay Kit

Item № 600050
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
96 wells $81.00 0.00
480 wells $304.00 0.00

Pricing updated 2018-02-19. Prices are subject to change without notice.

Description
Features
  • A simple fluorometric assay for the quantification of extracellular H2O2
  • Utilizes ADHP, a sensitive and stable probe for H2O2
  • Catalase, an H2O2 scavenger, is included as a control for specificity
  • Rapid assay; results in under 1 hour
Synonyms
  • H2O2 Fluorometric Assay Kit

Reactive oxygen species, such as hydrogen peroxide (H2O2) and superoxide are generated by phagocytes and participate in damaging invading miccroorganisms or other biologic targets.1 Cayman's H2O2 Cell-Based Assay Kit provides a simple fluorometric method for the sensitive quantitation of extracellular H2O2 produced by cultured cells. H2O2 is detected using ADHP (10-acetyl-3,7-dihydroxyphenoxazine), a highly sensitive and stable probe for H2O2.2 In the presence of horseradish peroxidase, ADHP reacts with H2O2 with a 1:1 stoichiometry to produce highly fluorescent resorufin.3 Resorufin fluorescence can be read using an excitation wavelength between 530-560 nm and an emission wavelength of 590 nm. Catalase, an H2O2 scavenger, is included in the kit for checking specificity of the assay.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • H2O2 Fluorometric Assay Kit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Rosen, G.M.,Pou, S.,Ramos, C.L., et al. Free radicals and phagocytic cells. The FASEB Journal 9, 200-209 (1995).

2. Zhou, M.,Diwu, Z.,Panchuk-Voloshina, N., et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: Applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Analytical Biochemistry 253, 162-168 (1997).

3. Amundson, D.M., and Zhou, M. Fluorometric method for the enzymatic determination of cholesterol. Journal of Biochemical and Biophysical Methods 38, 43-52 (1999).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of Hydrogen Peroxide Cell-Based Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640