A fluorometric or colorimetric assay for the quantification of H2O2 in cell culture supernatants
Features
  • A simple assay for the quantification of extracellular H2O2; measure fluorescence with an excitation at 530-560 nm and emission at 590 nm or absorbance at 570 nm
  • Utilizes ADHP, a sensitive and stable probe for H2O2
  • Catalase, an H2O2 scavenger, is included as a control for specificity
  • Rapid assay; results in under 1 hour
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Hydrogen Peroxide Assay Kit

Item No. 600050

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • H2O2 Fluorometric Assay Kit
Limit of Detection
0.02 µM (fluorometric); 0.15 µM (colorimetric)
Assay Range
0-10 µM (fluorometric); 0-80 µM (colorimetric)
Origin
Animal/Bovine, Plant/Armoracia rusticana
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $138

    Add

    480 wells

    $516

    Add
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    Product Description

    Cayman’s Hydrogen Peroxide Assay Kit provides fluorometric or colorimetric methods for the quantification of extracellular H2O2 produced by cultured cells. In the presence of horseradish peroxidase (HRP), H2O2 reacts stoichiometrically with an HRP substrate, ADHP, to produce the compound resorufin.1,2 Resorufin fluorescence can be easily quantified at excitation and emission wavelengths of 530-540 and 585-595 nm, respectively. Alternatively, the absorbance of resorufin can be measured at 570 nm. Catalase, an H2O2 scavenger, is included in the kit for verifying the specificity of the assay. ROS, such as H2O2 and superoxide, are generated by phagocytes and participate in damaging invading miccroorganisms or other biologic targets.3 This kit provides a valuable method for immunologists to assess the capacity of immune cell killing using ROS.

    This assay offers the option to measure absorbance or fluorescence. It is at the user’s discretion to choose the mode of measurement (and corresponding standard curve preparation) that best fits their needs. When read fluorometrically, this assay has a range of 0-10 µM and a lower limit of detection (LLOD) of 0.02 μM. When read colorimetrically, the range is 0-80 µM with an LLOD of 0.15 μM.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zhou, M., Diwu, Z., Panchuck-Voloshina, N., et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal. Biochem. 253(2), 162-168 (1997).

    2. Amundson, D.M., and Zhou, M. Fluorometric method for the enzymatic determination of cholesterol. J. Biochem. Biophys. Methods 38, 43-52 (1999).

    3. Rosen, G.M., Pou, S., Ramos, C.L., et al. Free radicals and phagocytic cells. The FASEB Journal 9, 200-209 (1995).