A convenient tool to quantify the cytotoxic effect of immune effector cells at a single cell level
Features
  • Label target cells with highly fluorescent CFSE for easy identification
  • Dead cells stain with 7-AAD
  • Analyze by flow cytometry to assess target cell killing
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7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit

Item No. 600120

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 7-Aminoactinomycin D/5(6)-Carboxyfluorescein Diacetate Succinimidyl Ester​
  • 7-Amino Actinomycin D/5(6)-Carboxyfluorescein Diacetate Succinimidyl Ester
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    1 ea

    $285

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    Product Description

    Cayman’s 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit employs CFSE to label target cells within the mixed cell population and 7-AAD to label dead cells. This kit provides an improvement over the traditional 51chromium (51Cr) release assay to assess cell-mediated cytotoxicity. CFSE labeling is more sensitive, does not employ radioisotopes, and cytolysis can be assessed at the single-cell level. The mixed lymphocyte reaction is a cornerstone method of immunology1, and this kit can help immunologists to detect subtle changes in cytotoxic lymphocyte function. The kit provides sufficient reagents to effectively stain approximately 109 cells.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Benczur, M., Petrányi, G.G., Pálffy, G., et al. Dysfunction of natural killer cells in multiple sclerosis: A possible pathogenetic factor. Clin. Exp. Immunol. 39, 657-662 (1980).

    Product Citations

    Guo, L., Yang, L., Speck, E.R., et al. Allogeneic platelet transfusions prevent murine T-cell-mediated immune thrombocytopenia. Blood 123(3), 422-427 (2014).